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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NUGC-4 is a human gastric cancer cell line derived from metastatic sites (paralympic lymph nodes) in patients with mixed gastric cancer (primarily signet ring cell carcinoma). It is widely used in gastrointestinal oncology to study the mechanisms of gastric cancer progression and metastasis. NUGC-4 cells are particularly renowned for their high potential for peritoneal metastasis, making them a primary model for peritoneal cancer research. This cell line exhibits epithelial cell morphology and is frequently used to evaluate the efficacy of novel chemotherapeutic drugs and targeted molecular therapies against gastric cancer. |
| Tissue | Stomach |
| Disease | Gastric Mixed-type Carcinoma; Signet Ring Cell Carcinoma |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | Not specified |
| Product Format | Frozen |
| Growth Mode | Adherent (can also grow in suspension or aggregates in certain media) |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Studies on gastric cancer metastasis and peritoneal dissemination 2. Signet ring cell carcinoma biology research 3. Gastric cancer drug screening and cytotoxicity assays 4. Tumor-stromal interaction and microenvironment studies 5. Construction of animal models of peritoneal metastasis |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and metastatic in nude mice |
| Genetic Profile | TP53 mutation; CDH1 (E-cadherin) alterations frequently observed |
| Expression Markers | Positive for epithelial markers; maintains characteristics of gastric secretory cells |
| Metastatic Potential | High potential for lymphatic and peritoneal spread |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Aliquot the cell suspension into new culture containers. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0638 | Human CLDN18-A2 Stable Cell Line - NUGC-4 | Inquiry |
| CSC-RO0973 | Human CLDN18-A2/Luc Stable Cell Line - NUGC4 | Inquiry |
| CSC-RR01007 | GFP/Luc Reporter Cell Line - NUGC-4 | Inquiry |
| CSC-RR0129 | GFP Stable Cell Line-NUGC4 | Inquiry |
| CSC-RR0130 | RFP Stable Cell Line-NUGC4 | Inquiry |
| CSC-RR0637 | Luciferase Reporter Cell Line - NUGC4 | Inquiry |
| CSC-RR0637-2 | tdTomato/Luc Reporter Cell Line - NUGC4 | Inquiry |
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