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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Caov-3 is a cell line with epithelial morphology that was isolated in 1976 from the ovaries of a 54-year-old Caucasian female patient with ovarian cancer. These cells form tightly packed colonies in adherent culture. All-trans retinoic acid has been shown to inhibit the in vitro growth of Caov-3 ovarian cancer cells. These cells express NB/70K, CA-125, Ba-2, and Ca-1 tumor-associated antigens. Caov-3 cells contain nonsense mutations in the p53 gene and have multiple copies of the ovarian cancer oncogene PIK3CA. They are sensitive to vinblastine, cisplatin, and doxorubicin. Although these cells will not grow in soft agar, they exhibit tumorigenic properties when injected into immunocompromised mice. Therefore, Caov-3 cells are particularly suitable for 3D cell culture experiments. |
| Tissue | Ovary |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 54 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and pharmacology 3. Molecular and genetic studies 4. Biological pathway analysis 5. Immunology studies 6. 3D cell culture |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Due to their epithelial morphology and ability to form dense colonies, Caov-3 cells are ideal for studying cell-cell interactions, tissue organization, and ovarian cancer cell behavior in a more physiological setting. However, the long doubling time of approximately 78 h must be considered in experimental design. |
| Characteristics | |
| Tumorigenic | Yes, in nude mice |
| Isoenzymes | AK-1, 1, ES-D, 1, G6PD, B, GLO-I, 1-2, Me-2, 2, PGM1, 1, PGM3, 1 |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Rinse the cell layer briefly with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently aspirate the cells. 5. Add an appropriate aliquot of the cell suspension to a new culture vessel. 6. Incubate the culture at 37℃. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | 90% DMEM + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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