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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | AGS cells are a human gastric adenocarcinoma cell line derived from gastric tissue of a 54-year-old Caucasian female. AGS cell lines exhibit epithelioid morphology, which is characterized by their invasive growth pattern and tumorigenic potential in vivo. These cells are often used as models to study the molecular and cellular mechanisms of gastric carcinogenesis, including the effects of Helicobacter pylori infection, a well-known risk factor for gastric cancer. AGS cells provide a powerful system to explore the interaction between gastric cancer cells and Helicobacter pylori, particularly with regard to how bacterial factors influence cancer cell proliferation, apoptosis, and inflammatory responses. |
| Tissue | Stomach |
| Disease | Gastric Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 54 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 |
| Applications | 1. Drug testing 2. Genetic studies 3. Disease modelling 4. Protein expression studies 5. Signal transduction research 6. Virus infection studies 7. 3D cell culture |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | AGS cells were persistently infected with parainfluenza virus type 5 (PIV5). This cell line has knockdown of the NOD1 gene and expresses DEFB4 in a NOD1-/cagPAI-dependent manner. These cells can be used to further study the role of NOD1 in H. pylori -induced gastric tumors. AGS cells are also valuable for examining the response of the gastric epithelial barrier to various stimuli, including inflammatory cytokines, and for studying signaling pathways related to gastric cancer, such as those involving NF-kB, Wnt, and MAPK. |
| Characteristics | |
| Cytogenetic Information | This is a hyperdiploid human cell line. The most common chromosome number is 49, found in 60% of cells. The polyploidy rate was 3.6%. |
| Protein Expression | p53 positive |
| Tumorigenic | Yes, in athymic BALB/c mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:6 is recommended. |
| Culture Medium | Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Cryopreservation Medium or complete growth media with 10% DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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