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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SGC-7901 is a widely used human epithelial cell line, originally established in 1979 from metastatic lymph nodes of a 56-year-old untreated Chinese woman with gastric adenocarcinoma. These cells exhibit typical epithelial morphology, appearing as rhomboid or polygonal shapes in monolayer cultures. Biologically, SGC-7901 serves as a fundamental model for studying the molecular mechanisms of gastric cancer progression and is widely used to investigate Helicobacter pylori-induced gastric epithelial inflammation. Notably, recent identification work has confirmed that SGC-7901 is a misidentified cell line, actually derived from the HeLa cervical adenocarcinoma cell line. Nevertheless, it remains a standard reference cell line in many published oncology and pharmacology studies. |
| Tissue | Stomach |
| Disease | Gastric Adenocarcinoma |
| Morphology | Epithelial-like; Polygonal; Rhomboidal |
| Gender | Female |
| Age | 56 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 |
| Applications | 1. Gastric cancer research 2. Drug screening and resistance 3. Inflammatory Signaling 4. In vivo xenograft modeling 5. Cancer stem cell studies |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Aneuploid; contains complex chromosomal rearrangements characteristic of transformed human lines. |
| Tumorigenic | Yes, forms vascularized solid tumors in immunocompromised mice. |
| Markers Expressed | Positive for epithelial membrane antigen (EMA) and Cytokeratin 20 (CK20); expresses various P2 purinergic receptors (P2X, P2Y). |
| Mutations | Presence of HPV-18 integrated sequences; alterations in Bcl-2/Bax ratios and COX-2 expression under stress. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T25 culture flasks) and observe under an inverted microscope until the cell layer disperses (usually 2 to 5 minutes). 4. Add an equal volume of complete culture medium to neutralize the trypsin. 5. Gently pipette the cells and transfer them to centrifuge tubes. 6. Centrifuge at 300 x g for 3 to 5 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers at the recommended seeding density. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI-1640 Medium + 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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|---|---|---|
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