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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Hep3B (Hep 3B2.1-7) is a cell line that exhibits epithelial morphology and was isolated from liver tissue of an 8-year-old black youth with hepatocellular carcinoma. Due to its widespread use in cancer research, the Hep3B cell line has become a well-characterized and widely available resource for researchers studying liver cancer and related diseases. It is also commonly used as a model system for studying other types of cancer, such as breast and lung cancer. Hep3B cells contain an integrated hepatitis B virus genome and are indispensable in studying differential drug responses due to their unique genetic and phenotypic characteristics. The Hep 3B human hepatoma cell line is known for its broad expression of liver-specific proteins, such as alpha-fetoprotein (AFP), albumin, and various other markers, making it a valuable tool in the study of drug metabolism and hepatotoxicity. This broad expression of proteins allows for a comprehensive assessment of how hepatoma cells interact with and metabolize therapeutic agents. |
| Tissue | Liver |
| Disease | Carcinoma; Hepatocellular |
| Morphology | Epithelial |
| Gender | Male |
| Age | 8 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Hepatocellular carcinoma studies 2. Genetic and epigenetic studies 3. Drug screening and development 4. Hepatitis B Virus research 5. Signal transduction studies 6. Protein expression and biochemistry 7. Gene therapy research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The Hep3B cell line (human liver) is used to create the CDX (cell line derived xenograft) Hep3B xenograft mouse model. The Hep3B xenograft model of human hepatocellular carcinoma (HCC) can be used to study tumor growth inhibition by therapeutic agents such as sorafenib, as well as to monitor studies for acquired sorafenib resistance. |
| Characteristics | |
| Karyotype | Modal number = 60 with a subtetraploid mode of 82; has a rearranged chromosome 1 |
| Protein Expression | Alpha Fetoprotein, Hepatitis B Surface Antigen (Hbsag), Albumin, Alpha2 Macroglobulin, Alpha1 Antitrypsin, Alpha1 Antichymotrypsin, Haptoglobin, Transferrin, Cerulopl |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Let the cells incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium. |
| Medium Renewal | Twice per week |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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