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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | The LL/2 (LLC1) Lewis lung carcinoma is a cell line established from the lungs of C57BL mice that develop tumors following implantation of primary Lewis lung carcinomas. The cells grow in semi-suspension culture, loosely attached to the surface of the culture vessel. The cell line is hypotetraploid with a chromosome modulus of 72. The tumorigenicity and metastatic properties of the cells are retained but reduced compared to the original in vivo tumor line. LL/2 cells have been widely used as a model system to study lung cancer biology, particularly in immunotherapy and the tumor microenvironment. LL/2 cells express lung cancer markers such as carcinoembryonic antigen (CEA) and cytokeratins, and exhibit several hallmarks of lung cancer, including uncontrolled proliferation, invasion, and metastasis. They are responsive to a variety of stimuli, such as cytokines and chemokines, making them a useful model system to study the effects of these factors on lung cancer cell growth and survival. |
| Tissue | Lung |
| Disease | Lewis Lung Carcinoma |
| Morphology | Rounded - loosely attached or floating |
| Strain | C57BL |
| Product Format | Frozen |
| Growth Mode | Mixed: adherent and suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug testing and development 3. Tumor growth and metastasis 4. Immunotherapy research 5. Genetic and molecular studies 6. In vivo studies 7. Radiation research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The LL/2 cell line is used to create the CDX (Cell Line Derived Xenograft) LL/2 xenograft mouse model. The LL/2 xenograft model, known to be resistant to BCNU, enables anti-tumor growth studies such as those studying inhibitors of BMK1 (e.g. XMD8-92). |
| Characteristics | |
| Antigen Expression | H-2b |
| Tumorigenic | Yes, in C57BL mice. |
| Viruses | MAP-test negative: Sendai, Ektromelia, Polyoma, Reo 3, PVM, LCM, M.pulmonis, MVM, Toolan's H-1, K-Virus, Kilham, MHV, LDV, RCV/SDA, Theiler's GD VII, M-Adenovirus, B.piliformis. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Collect the suspended cells into a 15 ml tube and gently wash the adherent cells with PBS without calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). 2. Ensure complete coverage of the cell layer using Accutase (use 1-2 ml for T25 flasks and 2.5 ml for T75 flasks). 3. Allow the cells to incubate for 10 minutes at room temperature. 4. After incubation, combine the suspended and adherent cells and centrifuge. 5. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension to a new flask containing fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
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