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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BxPC-3 (BxPC3) is a human pancreatic cancer cell line used to study pancreatic adenocarcinoma and its treatment. BxPC-3 cells were obtained from a 61-year-old female in 1986 and were confirmed to be tumorigenic in athymic nude mice with a moderate degree of differentiation. The BXPC-3 cell line has several properties that make it a useful tool in cancer research. It was able to grow in culture and exhibited many of the same properties as pancreatic cancer cells found in patients. Researchers used the BXPC-3 cell line to study the molecular mechanisms of pancreatic cancer, including genes and pathways involved in cancer cell growth, invasion and metastasis. The BXPC-3 cell line has also been used to test the efficacy of various cancer treatments. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 61 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Cancer biology research Drug screening and discovery Radiation therapy research Biomarker discovery |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | BxPC-3 cells lack KRAS mutations, although this mutation is common in pancreatic cancer. Both BcPC-3 cells and JoPaca-1 cells highly expressed cancer stem cell markers. It will be particularly useful in developing drugs that target the epidermal growth factor receptor (EGFR) and mitogen-activated protein kinase (MAPK) signaling pathways, which are frequently dysregulated in pancreatic cancer. |
| Characteristics | |
| Karyotype | 2n = 59, near triploid |
| Tumorigenic | Yes, nude mice inoculated subcutaneously with 1×107 cells developed tumors with 100% frequency within 21 days. |
| Genes Expressed | Mucin; pancreas cancer-specific antigen; carcinoembryonic antigen (CEA) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI 1640 + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
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|---|---|---|
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