RKO Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The RKO cell line is a human colon cancer cell line that is commonly used in cancer research. It was derived from a tumor of a 63-year-old male patient with colon adenocarcinoma. They are derived from a moderately differentiated colon adenocarcinoma and are notable for their wild-type p53 status, which is uncommon in many cancer cell lines. This property makes RKO cells particularly valuable for studying p53 function and the cellular mechanisms of DNA repair and apoptosis in colorectal cancer. The RKO cell line is tumorigenic in nude mice and expresses the urokinase receptor (u-PAR). They are widely used to study cancer biology, including cancer cell signaling, apoptosis, and drug resistance. Their sensitivity to chemotherapeutic drugs and radiation makes them suitable for use in drug discovery and development, helping to identify potential therapeutic targets and evaluate new treatment strategies for colorectal cancer.
Tissue	Colon
Disease	Carcinoma
Morphology	Epithelial
Gender	Male
Age	63 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Drug Testing and Development 3. Genetic Studies 4. Molecular Pathway Analysis 5. Biomarker Identification 6. Immunotherapy Research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The RKO cell line was used to create the CDX (cell line derived xenograft) RKO xenograft mouse model. The RKO xenograft model enables in vivo efficacy of immunotherapies such as pembrolizumab, ipilimumab and other targeted therapies such as NOB1 inhibition.
Characteristics
Oncogene	p53+ (wild-type)
Expression Markers	Urokinase receptor (u-PAR)
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes used in this protocol are for 75 cm² flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the culture medium. 2. Rinse the cell layer briefly with a 0.25% (w/v) trypsin-0.53% (w/v) EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to dissociate. Cells that are difficult to dissociate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette out the cells. 5. Add an appropriate amount of the cell suspension to a new culture vessel. 6. Incubate at 37℃.
Medium Renewal	Every 2 to 3 days.
Subcultivation Ratio	The ratio of 1:3 to 1:12 is recommended.
Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0172	Cas9 Stable Cell Line - RKO	Inquiry
CSC-RR00651	GFP Reporter Cell Line - RKO	Inquiry
CSC-RR00707	Luciferase Reporter Cell Line - RKO	Inquiry
CSC-RR01049	mScarlet/LUC Reporter Cell Line - RKO	Inquiry
CSC-RR0390	p53 Luciferase Reporter Cell Line - RKO	Inquiry

* For research use only. Not intended for any clinical use.

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