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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | EA.hy926 cells are a somatic hybrid cell line widely used in cardiovascular disease research. They are used to study various aspects of endothelial cell function related to angiogenesis, homeostasis/thrombosis, blood pressure regulation, and inflammation. The cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles in EA.hy926 cells observed by electron micrographs reflects their differentiated endothelial cell function. A key advantage of EA.hy926 cells is their ability to undergo more than 100 population doublings (PDLs) while maintaining their cellular properties. This long lifespan ensures a sustainable and consistent source of cells for long-term experiments and studies. With a doubling time of 12 hours, these cells exhibit rapid proliferation, facilitating experimental workflows and enabling efficient generation of cell numbers required for large-scale studies. |
| Morphology | Endothelial |
| Gender | Male |
| Tissue | Umbilical |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Vascular biology studies 2. Angiogenesis research 3. Drug testing and development 4. Inflammation and immune response studies 5. Blood-brain barrier research 6. Metabolic and cardiovascular research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The EA.hy926 cell line is a hybrid cell line generated by the fusion of HUVEC and A549 lung cancer cells. Primary HUVECs are characterized by limited lifespan, difficulty in maintenance, and large batch-to-batch variability, which can affect the reproducibility of results. On the other hand, immortalized EA.hy926 cells grow faster, are easier to handle, and produce reproducible results. |
| Characteristics | |
| Derivation | The human umbilical vein cell line EA.hy926 was established by fusing primary human umbilical vein cells with thioguanine-resistant A549 clone cells after polyethylene glycol (PEG) treatment. Hybrid clones were selected in HAT medium and screened for factor VIII-related antigens. |
| Antigen Expression | Factor VIII-related antigen; Homo sapiens, expressed |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for 75 cm2 flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the medium. 2. Briefly rinse the cell layer with Ca++/Mg++-free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) trypsin - 0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 ml of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 ml of complete growth medium and gently aspirate the cells. 5. Transfer the cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard the supernatant. 6. Resuspend the cell pellet in fresh growth medium. Add an appropriate amount of the cell suspension to a new culture vessel. The recommended inoculum size is 2 X 103 to 3 X 103 viable cells/cm2. 7. Incubate the culture at 37℃. Subculture when the cell concentration reaches 8 X 104 and 1 X 105 cells/cm2. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | 1:2 to 1:4 |
| Culture Medium | 90% DMEM + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 50% DMEM + 40% FBS + 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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