Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The PC3 (PC-3) cell line is a widely used cancer cell line derived from metastatic prostate adenocarcinoma. The PC3 cell line was established in 1979 from grade IV prostate cancer bone metastases in a 62-year-old white man. These cells did not respond to androgens, glucocorticoids, or fibroblast growth factor, but the results showed that these cells were affected by epidermal growth factor. It is known for its aggressive and highly tumorigenic properties, making it a valuable tool for studying prostate cancer biology and developing new treatments. |
| Tissue | Prostate |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 62 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | PC3 cell is a human prostate cancer cell line used in prostate cancer research and drug development. PC3 cells can be used to study biochemical changes in advanced prostate cancer cells and assess their response to chemotherapy drugs. PC3 cells are also used to study viral infections in mammalian cells that exhibit immune responses. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | PC3 cells have high metastatic potential compared with DU145 cells, which have intermediate metastatic potential, and LNCaP cells, which have low metastatic potential. Comparison of protein expression in PC3, LNCaP, and other cells indicates that PC3 is characteristic of small cell neoendocrine carcinoma. |
| Characteristics | |
| Antigen Expression | HLA A1, A9 |
| Tumorigenic | Yes, PC3 cells can be used to create subcutaneous tumor xenografts in mice to study the tumor environment and therapeutic drug function. |
| Mycoplasma Test | Negative |
| Cytogenetic Information | Karyotype analysis showed that PC3 was close to triploid, with 62 chromosomes. Q-band analysis shows no Y chromosome. From a morphological point of view, electron microscopy showed that PC3 cells exhibited characteristics of poorly differentiated adenocarcinoma. |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | Coons Modified Ham's F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign's modified Ham's F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0608 | Human CD274 Stable Cell Line - PC3 | Inquiry |
| CSC-RO0850 | Human NECTIN4 Stable Cell Line - PC3 | Inquiry |
| CSC-RO0910 | Human CD274-TACSTD2 Stable Cell Line - PC3 | Inquiry |
| CSC-RO0915 | Human TACSTD2 Stable Cell Line - PC3 | Inquiry |
| CSC-RO1013 | Cas9 Stable Cell Line - PC3 | Inquiry |
| CSC-RR00704 | Luciferase Reporter Cell Line - PC-3 | Inquiry |
| CSC-RR01025 | GFP/Luc Reporter Cell Line - PC-3 | Inquiry |
| CSC-RR0116 | GFP Stable Cell Line-PC-3 | Inquiry |
| CSC-RR0286 | RFP Reporter Cell Line - PC3 | Inquiry |
| CSC-RT2632 | Human DLX1 Knockout Cell Line-PC3 | Inquiry |
| CSC-RT2634 | Human E2F1 Knockout Cell Line-PC3 | Inquiry |
| CSC-RT2770 | Human EGFR Knockout Cell Line-PC3 | Inquiry |
| CSC-RT2783 | Human NYNRIN Knockout Cell Line-PC3 | Inquiry |
| CSC-RT2784 | Human NDP Knockout Cell Line-PC3 | Inquiry |
| CSC-RT2809 | Human DCAF16 Knockout Cell Line-PC3 | Inquiry |
Copyright © Creative Biogene. All rights reserved.