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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | McA-RH7777 is a cell with epithelial-like morphology that was isolated from the liver of female rats with hepatocellular carcinoma. These cells exhibit adherent growth with a distinctive fusiform and polygonal appearance. They induce subcutaneous tumors in rats following subcutaneous injection of MCA-RH 7777. In vitro, McA-RH 7777 cells dynamically participate in the production and release of plasma lipoproteins. Notably, MCA-RH 7777 cells produce alpha-fetoprotein (AFP) and exhibit high levels of L-histidine decarboxylase (HDC) expression, leading to histamine (HA) synthesis. These cells express H3 receptor (H3R) transcripts and proteins, and their proliferation is regulated by HA through interaction with the autoinhibitory H3R. |
| Tissue | Liver |
| Disease | Hepatoma |
| Morphology | Epithelial |
| Strain | Buffalo |
| Gender | Female |
| Product Format | Frozen |
| Growth Mode | Loosely adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Gene Expression Studies 3. Drug Metabolism and Toxicity Testing 4. Lipid Metabolism Research 5. Transfection and Reporter Gene Assays 6. Signal Transduction Studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | McA-RH7777 cells have 2-fold higher fatty acid uptake and 2-fold lower fatty acid oxidation compared to primary hepatocytes. McA-RH7777 cells do not synthesize significant amounts of glycogen, but instead preferentially metabolize glucose to lipids or oxidation. In primary hepatocytes, glucose is largely not oxidized, but instead is divided between de novo glycogen and lipid synthesis. |
| Characteristics | |
| Genes Expressed | Alpha-fetoprotein (AFP, alpha fetoprotein) |
| Expression Markers | Glucocorticoid |
| Tumorigenic | Yes |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Thick monolayers of cells detach; subculture before 70% confluence. The volumes used in this protocol are for 75 cm2 flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Transfer the medium with floating cells to a centrifuge tube. 2. If cells are attached, gently tap the flask or add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution to the flask as needed and observe the cells under an inverted microscope until the cell layer is dispersed. 3. Add 2.0 to 3.0 mL of complete growth medium and gently aspirate the cells. 4. To remove the trypsin-EDTA solution, transfer the cell suspension along with the medium and cells from step #1 to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 5. Discard the supernatant and suspend the cells in fresh growth medium. Add an appropriate aliquot of the cell suspension to a new culture vessel. 6. Place the culture vessel in a 37℃ incubator. |
| Medium Renewal | Add medium every 2 to 3 days, do not discard floating cells. |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | EMEM + 20% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
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| CSC-RG1794 | Mouse LPAR1 Stable Cell Line - RH7777 | Inquiry |
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