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E.coli Expression System


Creative Biogene is a biotechnology company which has the expertise and experience in producing custom proteins...

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E.coli Expression System

Creative Biogene is a biotechnology company which has the expertise and experience in producing custom proteins by E.coli expression system. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the complete protein production and purification service, including sequence optimization and synthesis, recombinant plasmid construction, flexible scale protein production and purification, post processing of purified protein et al. Creative Biogene is confident in providing you with the most affordable, high-quality gene expression services using E.coli expression system in a short turnaround time.

E.coli is one of the most widely used expression hosts for the production of recombinant proteins. It is often the first system chosen for producing target proteins due to its advantages over other systems, such as rapid proliferation, high expression, easy purification, good stability and low cost. Although there are some difficulties in the E.coli expression system, such as codon usage bias in the target gene sequence or imbalance between protein aggregation and solubilization, which may lead to the failure of target protein expression/low expression or result in the formation of protein aggregates (commonly known as inclusion bodies). Creative Biogene possesses mature codon optimization system, refolding system and professional staffs which can not only ensure the expression quantity and purity of your protein, but also refold the inclusion bodies and thereby obtain soluble, bioactive recombinant proteins.

Applications:

  • Therapeutic antibodies for in vivo studies
  • Research tool proteins for crystallography
  • Large-scale protein production for feed studies

Features:

  • One-stop solution from gene synthesis/gene cloning to purified protein
  • Offer custom construction of expression vectors with different promoters or tags
  • High recombinant protein production levels
  • Short timeline from gene synthesis/cloning to protein recovery
  • Cost-saving: provide high quality proteins with competitive price

Creative Biogene provides gene expression service based on E.coli expression system, which includes:

  • Codon optimization for any given expression system (upon request)
  • Synthesis genes of interest with or without tags
  • Recombinant plasmid construction
  • Target protein expression and purification
  • Protein post processing (optional) such as purified protein tag removal, filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing et al.)

References:

  1. Gräslund S., et al. (2008). Protein production and purification. Nature methods, 5(2), 135-146.
  2. Sambrook J. et al. (1989). Molecular cloning: A Laboratory Handbook. 2. NOLAN, C. (ed.), 2.

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