293T Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The 293T cell line was created in the Michele Calos laboratory by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is able to replicate vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, DNA replication, transfection, gene expression, and protein production properties. It gives high titres when used to produce a number of viral vectors such as lentiviruses and oncoretroviruses.
Tissue of Origin	Kidney (foetal)
Cell Type	Small slightly rounded cells growing in a dispersed manner
Product Format	Frozen
Growth Mode	Adherent
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Volumes are given for a 75 cm² flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes. Remove and discard the culture medium. Briefly rinse the cell layer with Mg²⁺/Ca²⁺ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0-2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. And incubate cultures at 37°C.
Subcultivation Ratio	The ratio of 1:3 to 1:8 is recommended.
Fluid Renewal	Every 2-3 days
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Culture Temperature	37°C
Atmosphere	Air, 95%; carbon dioxide (CO₂)

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Cat.No.	Product Name	Price
CSC-RO01157	Cas9 Stable Cell Line - 293T	Inquiry
CSC-RR00656	Luciferase Reporter Cell Line - 293T	Inquiry
CSC-RT0108	POLR3B Knockout Cell Line-293T	Inquiry
CSC-RT0131	DCTPP1 Knockout Cell Line-293T	Inquiry
CSC-RT0142	NTPCR Knockout Cell Line-293T	Inquiry
CSC-RT0143	AMPD2 Knockout Cell Line-293T	Inquiry
CSC-RT0144	PAFAH1B3 Knockout Cell Line-293T	Inquiry
CSC-RT0145	CELF1 Knockout Cell Line-293T	Inquiry
CSC-RT0146	RDX Knockout Cell Line-293T	Inquiry
CSC-RT0147	MYL6B Knockout Cell Line-293T	Inquiry
CSC-RT0148	PLEKHJ1 Knockout Cell Line-293T	Inquiry
CSC-RT0149	PFDN1 Knockout Cell Line-293T	Inquiry
CSC-RT0150	UBXN6 Knockout Cell Line-293T	Inquiry
CSC-RT0151	MAT2B Knockout Cell Line-293T	Inquiry
CSC-RT0152	HCFC1R1 Knockout Cell Line-293T	Inquiry

* For research use only. Not intended for any clinical use.

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