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293T Cell Line


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293T Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionThe 293T cell line was created in the Michele Calos laboratory by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is able to replicate vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, DNA replication, transfection, gene expression, and protein production properties. It gives high titres when used to produce a number of viral vectors such as lentiviruses and oncoretroviruses.
Tissue of OriginKidney (foetal)
Cell TypeSmall slightly rounded cells growing in a dispersed manner
Product FormatFrozen
Growth ModeAdherent
Biosafety Level2 [Cells contain Adenovirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipped inDry ice
Storage Temperature−196°C
ReferencesDuBridge RB, et al. 1987 Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol Cell Biol. 7(1):379-87.
Characteristics
Images293T Cell Line
CommentsThis cell line is able to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses. It has been extensively used for retroviral production, gene expression and protein production.
MycoplasmaNegative in microbiological culture, PCR assays
ImmunologyCytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, endothel -, EpCAM -, GFAP -, neurofilament -, desmin -, vimentin +
FingerprintSame DNA profile as 293 (ACC 305) by fluorescent nonaplex PCR of short tandem repeat markers
VirusesPCR: HBV -, HCV -, EBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -, MLV -
Culture Conditions and Handling
Culture MediumThe base medium for this cell line is Dulbecco’s Modified Eagle’s Medium (DMEM). To make the complete growth medium, add the following components to the base medium: 10% Fetal Bovine Serum (heat inactivated), 1% Penicillin/Streptomycin, 2 mM L-glutamine.
SubculturingVolumes are given for a 75 cm2 flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard the culture medium.
  2. Briefly rinse the cell layer with Mg2+/Ca2+ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0-2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5-15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal.
  4. Add 6.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. And incubate cultures at 37°C.
Subcultivation RatioThe ratio of 1:3 to 1:8 is recommended.
Fluid RenewalEvery 2-3 days
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereAir, 95%; carbon dioxide (CO2)
Cat.No. Product Name Price
CSC-RG0167Human ADORA2B-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0168Human ADORA3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0176Human ADRB3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0177Human C3AR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0178Human C5AR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0185Human NMBR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0186Human GRPR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0187Human BRS3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0188Human BDKRB1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0189Human CALCRL-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0190Human CASR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0193Human CNR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0196Human GPR55-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0197Human CCR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0198Human CCR10-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0199Human CCR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0200Human CCR3-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0201Human CCR3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0202Human CCR4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0203Human CCR5-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0205Human CCR6-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0206Mouse Ccr6-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0207Human CCR7-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0208Human CCR8-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0210Human CCR9-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0211Human CCR9-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0213Mouse Ccr9-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0214Mouse Ccr9-FLAG Stable Cell Line-HEK293T Gqi5Inquriy
CSC-RG0215Human CMKLR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0217Human CX3CR1-FLAG Stable Cell Line-HEK293T Gqi5Inquriy
CSC-RG0218Human CX3CR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0219Human CXCR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0220Human CXCR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0221Human CXCR3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0222Human CXCR4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0224Human CXCR5-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0227Human CXCR6-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0228Human CXCR7-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0229Human XCR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0232Human CCKAR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0235Human CRHR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0236Human CRHR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0237Human DRD2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0239Human DRD4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0240Human DRD5-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0242Human GPR119-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0246Human O3FAR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0247Rat O3far1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0248Mouse O3far1-FLAG Stable Cell Line-HEK293T, chimeric G protein Galphaqi5Inquriy
CSC-RG0249Human FFAR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0251Rat Ffar1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0252Mouse Ffar1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0254Human FFAR3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0256Human FFAR3-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0258Human FFAR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0262Human GALR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0264Mouse Ghsr-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0265Human GHSR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0267Human GHRHR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0268Human GIPR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0271Rat Tshr Stable Cell Line-HEK293TInquriy
CSC-RG0272Human GNRHR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0273Human HRH1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0274Human HRH2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0275Human HRH4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0276Mouse Hrh4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0279Human FPR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0280Human LTB4R2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0281Human CYSLTR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0282Human CYSLTR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0294Rat Mchr1 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0295Human MCHR1 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0297Human MC4R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0299Human MTNR1B Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0300Mouse Mtnr1b-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0303Human GRM2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0304Human GRM3-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0305Rat Grm4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0306Human GRM4-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0307Human GRM4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0308Human GRM5-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0309Human GRM6-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0310Human GRM7-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0311Human GRM7-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0312Human GRM8-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0314Human GRM8-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0322Human FPR1-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0323Human NMUR2-FLAG Stable Cell Line-HEK293T (one sense mutation, which has been noted in literature, in comparison to GenBank Accession Number NM_020167.3)Inquriy
CSC-RG0324Mouse Npsr1 Stable Cell Line-HEK293TInquriy
CSC-RG0325Human NPSR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0326Human NPSR1 variant 2 Stable Cell Line-HEK293TInquriy
CSC-RG0327Human NPSR1 variant Asn107Ile cDNA (SNP591694 A>T; refSNP ID rs324981) Stable Cell Line-HEK293TInquriy
CSC-RG0328Human NPBWR1 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0329Rat Npbwr1 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0330Human NPBWR2 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0335Human NPY5R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0336Human NPY5R-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0343Human OPRK1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0346Human OPRL1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0351Human GPR3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0352Human GPR37L1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0354Human MRGPRX2 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0359Human PTGER1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0360Human PTGER2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0366Human PTGER4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0367Human PTGFR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0369Human TBXA2R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0376Human P2RY14-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0377Human P2RY14-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0381Human RXFP1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0382Human RXFP3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0383Human HTR1A-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0384Human HTR1B-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0385Dog HTR2A-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0387Human HTR2B-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0391Human HTR4-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0393Human HTR7-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0394Human SMO-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0405Human TACR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0406Human TACR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0407Human TACR3-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0408Rat Taar1 Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0409Human TAAR1 Stable Cell Line-HEK293TInquriy
CSC-RG0417Human GPR39-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0418Human AGTR1-FLAG Stable Cell Line-HEK293T (same as GenBank Accession Number NM_000685.4 except for one silent mutation)Inquriy
CSC-RG0421Human BDKRB2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0422Human CALCR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0424Rat Casr-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0425Human CASR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0427Human CCKBR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0429Human DRD1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0433Human O3FAR1-FLAG Stable Cell Line-HEK293T Galphaqi5Inquriy
CSC-RG0441Human GLP1R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0442Human GCGR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0443Human TSHR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0445Human LTB4R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0452Human GRM2-FLAG Stable Cell Line-HEK293T Gqi5Inquriy
CSC-RG0457Human GRM6-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0460Human MLNR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0461Human NMUR1-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0462Human PTH2R-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0463Human PTAFR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0464Human PTGDR-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG0465Human PTGDR2-FLAG Stable Cell Line-HEK293TInquriy
CSC-RG1717Rat ADORA2B Stable Cell Line-HEK293TInquriy
CSC-RG1718Human CCR1 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1719Human CCR7 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1720Human CCR8 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1721Dog CCR9 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1722Rat CCR9 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1723Human CXCR1 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1724Human CXCR2 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1725Human CXCR3 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1726Human CXCR4 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1727Human CXCR6 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1728Human CRHR1 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1731Rat FFAR3 Stable Cell Line-HEK293TInquriy
CSC-RG1732Rat FFAR2 Stable Cell Line-HEK293TInquriy
CSC-RG1733Human HRH3 Stable Cell Line-HEK293TInquriy
CSC-RG1734Mouse Hrh3 Stable Cell Line-HEK293TInquriy
CSC-RG1736Human HRH4 Stable Cell Line-HEK293T Gαqi5Inquriy
CSC-RG1740Human GPR35 Stable Cell Line-HEK293TGα16Inquriy
CSC-RG1741Human GPR50 Stable Cell Line-HEK293TInquriy
CSC-RG1742Human GPR142 Stable Cell Line-HEK293TInquriy
CSC-RG1744Human PTH1R Stable Cell Line-HEK293TInquriy
CSC-RG1747Human ADCYAP1R1 Stable Cell Line-HEK293TInquriy
CSC-RN0001Human ERR alpha (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0003Human FXR (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0006Human GR (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0009Human LXR alpha (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0011Human LXR beta (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0013Human MR (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0014Human PPAR alpha (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0016Human PPAR delta (LBD)-GAL4 (DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0018Human PPAR gamma(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0020Human PR(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0022Human RAR alpha(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0024Human RAR beta(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0026Human RAR gamma(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0028Human ROR gamma(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla) (tetracycline-inducible)Inquriy
CSC-RN0030Human RXR alpha(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0032Human RXR beta(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0034Human TR alpha(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0036Human TR beta(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RN0038Human VDR(LBD)-GAL4(DBD) Stable Cell Line-HEK 293T(UAS-bla)Inquriy
CSC-RR0303DDX58/Luc Reporter Cell Line-HEK293TInquriy
CSC-RR0304IFIH1/Luc Reporter Cell Line-HEK293TInquriy
CSC-RG1633Human AGTR1 Stable Cell Line-HEK293TInquriy
CSC-RG1638Human NMUR2 Stable Cell Line-HEK293TInquriy
CSC-RR0043GFP-Puro Reporter Stable Cell Line-HEK293TInquriy
CSC-RR0220PKC-Luc Reporter Cell Line-HEK293TInquriy
CSC-RR0221NFAT-Luc Reporter Cell Line-HEK293TInquriy
CSC-RR0222TCF/LEF-Luc Reporter Cell Line-HEK293TInquriy
CSC-RT0108POLR3B Knockout Cell Line-293TInquriy
CSC-RT0131DCTPP1 Knockout Cell Line-293TInquriy
CSC-RT0142NTPCR Knockout Cell Line-293TInquriy
CSC-RT0143AMPD2 Knockout Cell Line-293TInquriy
CSC-RT0144PAFAH1B3 Knockout Cell Line-293TInquriy
CSC-RT0145CELF1 Knockout Cell Line-293TInquriy
CSC-RT0146RDX Knockout Cell Line-293TInquriy

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