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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | LN-229 is a cell line that exhibits epithelioid morphology and was isolated in 1979 from the right fronto-parieto-occipital cortex of a 60-year-old white female patient with glioblastoma. The LN-229 cell line has the wild-type PTEN gene, mutated p53, and possibly homozygous deletions in p16 as well as in the p14ARF tumor suppressor gene. In a 1997 study, the LN-229 cell line was evaluated following treatment with puromycin, which killed LN-229 cells in a dose-dependent manner. Furthermore, stimulation of these cells with Fas ligand resulted in apoptosis within 16 h. |
| Tissue | Brain; Right frontal parieto-occipital cortex |
| Disease | Glioblastoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 60 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Studying cell signaling pathways Neuroscience research Drug screening and development Tumor biology research |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Bcl-2 protects these LN229 cells from Fas ligand-induced cell death but has only a minimal protective effect against puromycin-induced apoptosis. |
| Characteristics | |
| Oncogene | p53+ (mutated, CCT (Pro) --> CTT (Leu) mutation at codon 98); PTEN+ (wild-type); p16- (deleted); p14ARF- (deleted) |
| Tumorigenic | Yes, forms tumors in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to the flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal. 4. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:5 is recommended. |
| Culture Medium | DMEM+5% FBS+1% P/S |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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|---|---|---|
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