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Ion channels are important components of cells and play a vital role in various body functions. These tiny pores found in cell membranes allow specific ions to flow in or out of the cell, thereby regulating a wide range of physiological processes. Ion channels are involved in the transmission of nerve impulses, maintaining cell electrical potential, muscle contraction, and the release of neurotransmitters. Their dysfunction can lead to various diseases, making them important targets for medical research.
To obtain more specific biophysical and pharmacological analysis, scientists simplify ion channels by expressing it in cultured, non-neuronal cell lines. Creative Biogene has the most extensive collection of cell lines stably expressing functional ion channels, including Nav, Cav, ERG, and others. These cell lines are carefully selected for optimal performance thus ensuring a high level of accuracy and precision in the assays. Our experienced scientists will provide researchers with extensively optimized and validated ion channel stable cell lines for your electrophysiology experiments.
Creative Biogene has accesioned a lare collection of cell lines that stably express high levels of potassium channels.
These sodium channel stable cell lines have excellent in vitro assay sensitivity and reproducibility.
These TRP ion channel stable cell lines enable the assssment of ion channel function by detection electrophysiological signals.
Creative Biogene has accessioned a large collection of calcium channel cell lines to aid in the study of calcium channels.
Creative Biogene has accessioned a large collection of chloride channel cell lines to aid in the study of chloride channels.s.
Creative Biogene has accessioned a large collection of HCN channel cell lines to aid in the study of chloride channels.
These ligand-gated ion channel stable cell lines can be used for screening ion channel inhibitors.
Ion channels are an important component of cell membranes and play a vital role in maintaining homeostasis and regulating cellular processes. They facilitate the movement of ions in and out of cells, which are critical for functions such as signal transduction, electrical signaling, and cell volume regulation. Research into the behavior of ion channels and their dysregulation in disease has led to the development of stable cell lines expressing specific ion channels, allowing researchers to study their function and potential therapeutic applications. The stable expression of ion channels in these cell lines allows the study of their activation, regulation and functional properties in a standardized and reproducible manner.
Applications for ion channel stable cell lines include:
Case Study 1
In the TRPV1-overexpressing HEK293T cell line, researchers found that troxerutin antagonized the calcium influx induced by the TRPV1 agonist capsaicin in vitro.
Figure 1. Capsaicin-induced calcium influx in TRPV1-overexpressing HEK293T cells. (TRPV1-overexpressing HEK293T cell line was purchased from Creative Biogene) (Lee J, et al. 2023)
Case Study 2
Glioblastoma multiforme is the most common malignant primary brain tumor. Here, researchers show that in a patient-derived orthotopic xenograft (PDOX) model and in patient glioblastoma tumors, KCNA1 is overexpressed in GBMiinv cells in vivo and acts as a downstream target of microRNA in GBM cells to promote tumor invasion and metastasis. KCNA1 may promote tumor growth and invasion by upregulating the expression of SLC7A11 and inhibiting ferroptosis, making it a promising therapeutic target for GBM.
Using CCK-8 assays and colony formation experiments, the researchers demonstrated that KCNA1 enhances the proliferative capacity of GBM cells. Western blot analysis was used to verify the increased expression of proteins associated with tumor invasion in KCNA1 overexpressing (KCNA1-OE) cells (Figure 2. E). Furthermore, researchers verified that KCNA1 overexpressing (KCNA1-OE) cells exhibited enhanced invasion and migration abilities in 2D and 3D models (Figure 2. F, G).
Figure 2. KCNA1 promotes GBM progression in vitro. (Gombos R B, et al., 2018)
Q: What are ion channels?
A: Ion channels are membrane proteins that allow ions to pass through the cell membrane. They play a crucial role in regulating the flow of ions, such as sodium (Na+), potassium (K+), calcium (Ca2+), and chloride (Cl-), into and out of cells. Ion channels have a selective permeability, allowing specific ions to move across the membrane while blocking others.
Q: What factors affect the opening and closing of ion channels?
A: The opening and closing of ion channels are controlled by various factors, such as voltage, ligands, mechanical stress, or temperature changes.
Q: How are ion channels linked to diseases?
A: The malfunction of ion channels can lead to various diseases. For instance, dysfunction of potassium channels can cause cardiac arrhythmias and epilepsy while dysregulation of calcium channels has been implicated in neurodegenerative disorders, such as Alzheimer's disease.
Q: What are ion channel stable cell lines?
A: Ion channel stable cell lines are genetically modified cell lines that express specific ion channels at the cell membrane and provide a controlled environment for studying the properties and mechanisms of ion channels.
Q: What are the applications of ion channel stable cell lines?
A: By using ion channel stable cell lines, researchers can investigate the role of specific ion channels in cellular physiology and disease, test the effects of different drugs or compounds on ion channel function, and screen large compound libraries to identify potential therapeutics that modulate or target specific ion channels.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RG01846 | Human GLP1R Stable Cell Line - HEK293 | Inquiry |
| CSC-RI0001 | Human CACNA1B Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0002 | Human CACNA1H Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0003 | Human CFTR Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0004 | Human GABRA1 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0005 | Human GABRA2 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0006 | Human GABRA3 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0007 | Human GABRA4 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0008 | Human GABRA5 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0009 | Human GABRA6 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0010 | Human GLRA1 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0011 | Human HCN1 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0012 | Human HCN2 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0013 | Human HCN3 Stable Cell Line-HEK293 | Inquiry |
| CSC-RI0014 | Human HCN4 Stable Cell Line-CHO-K1 | Inquiry |
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