Phosphatase Screening & Profiling Services

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Overview

Protein phosphorylation and dephosphorylation, coordinated by kinases and phosphatases, are central to signal transduction, metabolism, immunity, and cell-cycle regulation. While kinases have long dominated drug discovery, the critical roles of phosphatases—including serine/threonine phosphatases and protein tyrosine phosphatases—are increasingly recognized in cancer, immunology, neurology, and metabolic diseases. Advances in structural and chemical biology have revealed their diverse catalytic mechanisms, allosteric regulation, and subtype-specific conformations, creating new therapeutic opportunities and driving demand for reliable, high-quality assays to assess compound potency and selectivity.

Creative Biogene provides an integrated Phosphatase Screening & Profiling Service platform, enabling rapid, reproducible, and mechanistically informed evaluation of small molecules against a broad panel of phosphatases. Our capabilities span Ser/Thr phosphatases, classical PTPs, dual-specificity phosphatases, and disease-relevant mutant phosphatases. With deeply optimized fluorescence-based biochemical assays, automated high-throughput pipelines, and expert data interpretation, we support early-stage hit identification, selectivity profiling, and lead optimization with high scientific rigor.

Our Service Portfolio

Serine/Threonine Phosphatase Screening & Profiling

Ser/Thr phosphatases regulate diverse pathways, including cell-cycle control, stress responses, and metabolic regulation. We offer assay systems covering major families such as:

PP1, PP2A, PP2B (calcineurin)
PP2C, PP4, PP5, PP6
Special substrates or pathway-customized assays upon request.

Assays quantitatively evaluate compound effects on catalytic turnover, substrate dephosphorylation, and dose-dependent inhibitory activity. These services support early discovery efforts for oncology, immunology, and neurobiology programs.

Protein Tyrosine Phosphatase (PTP) Screening & Profiling

Creative Biogene provides a robust panel of classical and non-classical PTPs, suitable for mechanism exploration, hit validation, and selectivity ranking:

Classical PTPs: PTP1B, SHP2, TC-PTP, CD45, LAR
Dual-Specificity Phosphatases (DUSPs): DUSP1, DUSP6, MKPs
Low-molecular-weight PTPs
Mutant or disease-associated variants such as SHP2-E76K

All assays are optimized for low background noise, linear reaction kinetics, and reproducible IC₅₀ determination.

Assay Technology and Screening Strategy

Creative Biogene leverages deeply optimized, fluorescence-based biochemical assays, designed to capture phosphatase activity under physiologically relevant conditions. Depending on the enzyme family, we employ:

Fluorogenic phosphopeptide substrates
High-sensitivity phosphate-release detection
Kinetic measurements to capture loop dynamics or allosteric effects
Single-concentration HTS and full dose–response profiling
Orthogonal validation assays when required

Assays are executed in 96-, 384-, or 1536-well formats, enabling rapid screening of focused libraries or larger compound sets with minimal material requirements.

Advantages of Creative Biogene's Phosphatase Profiling Platform

Extensive Assay Library

Over 20+ validated phosphatase assays, continuously expanding based on client demand and scientific advancements.

High Reproducibility and Low Artifact Rates

Assay conditions are optimized to minimize false positives/negatives, supported by stringent QC, internal controls, and statistical quality metrics.

Experienced Scientific Team

Our enzymology and cell-signaling specialists bring years of experience in phosphatase structural biology, assay design, and small-molecule evaluation.

Flexible Throughput Options

From rapid single-point screens to detailed dose-response curves and IC₅₀ determination, we tailor the workflow to your project stage.

Mechanistic Interpretation

Data are analyzed by experts who understand phosphatase biology, enabling meaningful interpretation beyond raw activity numbers.

Service Workflow

Although each project is customized, most follow a clear and efficient process:

1Project Consultation & Enzyme Selection: Assist in choosing phosphatase targets, assay formats, and screening scope.

2Assay Setup & Qualification: Optimize enzymes, substrates, buffers, and detection for reliable performance.

3Screening or Profiling Execution: Perform HTS, focused screens, or selectivity panels on automated platforms.

4Data Analysis & Quality Assessment: Conduct curve fitting, IC₅₀ calculation, Z' evaluation, and artifact filtering.

5Reporting & Scientific Discussion: Deliver results with data, plots, and interpretation, with follow-up for pipeline integration.

Start Your Phosphatase Project

If your research involves phosphatase inhibitor discovery, selectivity assessment, or mechanistic characterization, Creative Biogene can provide a customized and efficient screening solution. Contact us to discuss your project needs — our team is ready to support your next discovery milestone.

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