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Double-stranded RNA (dsRNA) consists of two complementary RNA strands forming a double helix. It acts as a key trigger molecule in the RNA interference (RNAi) pathway—processed by Dicer/RNase III enzymes into short interfering RNAs (siRNAs) that guide RISC complexes to degrade or suppress target mRNA. Beyond gene silencing, dsRNA has broad applications in chromatin regulation, antiviral defense, pest management, plant breeding, and diagnostic development.
Figure 1. Schematic of human genomic repeats linked to dsRNA formation. (Sadeq S, et al., 2021)
As research and agricultural RNAi technologies expand, there is growing demand for long, high-quality dsRNA at reasonable cost. Creative Biogene's synthesis platform addresses this need with optimized in vitro transcription systems, flexible labeling options, and stringent QC control.
Creative Biogene offers dsRNA synthesis services, supporting research and industrial applications in RNA interference (RNAi), gene silencing, pest control, plant protection, and molecular imaging. With advanced RNA synthesis platforms and years of RNAi expertise, we provide high-quality, customizable dsRNA products ranging from 150 bp to 2,000 bp, available in standard or fluorescently labeled forms, ensuring exceptional purity, structural integrity, and reproducibility.
Exceptional Purity and Structural Integrity
Comprehensive removal of DNA templates, proteins, salts, and ssRNA contaminants guarantees high-purity dsRNA with excellent duplex stability. All operations are performed under RNase-free conditions to maintain molecular integrity throughout production.
Customizable Labeling and Modification Options
Terminal or internal fluorescent labeling (FAM, Cy3, Cy5, etc.) enables direct imaging, quantitative tracking, and functional studies. Additional chemical or probe modifications can be incorporated according to research or screening requirements.
Rapid Turnaround with Cost Efficiency
Optimized synthesis and purification pipelines support fast delivery—typically within 1–4 weeks depending on fragment length and labeling complexity—at highly competitive prices suitable for both research and pilot-scale production.
1Project Consultation: Define sequence, length, modification, and yield requirements.
2Template Design & Optimization: Adjust GC content and structure; construct T7 or dual-promoter templates.
3In Vitro Transcription & Annealing: Synthesize and hybridize complementary strands under optimized conditions.
4Purification & QC: Purify by PAGE or HPLC; validate integrity, purity, and labeling efficiency.
5Delivery & Support: Provide QC report, storage guidance, and technical assistance.
| Parameter | Typical Range / Option | Notes |
| dsRNA length | 150–2,000 bp | Longer fragments available upon request |
| Format | Pre-annealed, labeled/unlabeled, powder or solution | Customizable |
| Purity | DNA-/protein-/rNTP-free | PAGE or HPLC optional |
| Yield | µg–mg scale | Depends on sequence and modifications |
| Labeling efficiency | ≥80–90% (for fluorescent tags) | Verified by fluorescence |
| QC report | Included | Gel, UV, purity, labeling data |
As dsRNA technology continues to expand across functional genomics, agriculture, diagnostics, and molecular therapeutics, Creative Biogene remains committed to advancing synthesis precision, labeling diversity, scalability, and stability enhancement.
Whether you need small-scale research-grade dsRNA or bulk production for field application, our experts will design and deliver the optimal solution for your project. Contact us today with your target sequence, application, and specifications, and let Creative Biogene accelerate your RNAi research and innovation with reliable, high-performance dsRNA synthesis.
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