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● In contemporary genomics and molecular genetics research, Bacterial Artificial Chromosome (BAC) libraries have become a critical bridge connecting gene function analysis with chromosomal structure studies. BACs are vectors derived from the F-plasmid replication system, characterized by a low copy number, high stability, and the capacity to carry large DNA inserts. Each BAC clone can accommodate DNA fragments ranging from 150 to 300 kb, far exceeding the capacity of standard plasmid or λ-phage vectors. This allows the preservation of regulatory elements, repetitive sequences, and long-range structural regions, enabling chromosome-level studies.
Figure 1. The pipelines of BAC end libraries and BAC-anchor analysis. (Yang X, et al., 2019)
Key advantages of BAC libraries include:
Creative Biogene has long been committed to constructing large-scale DNA libraries and advancing their applications. Leveraging advanced molecular cloning technologies and a mature screening system, we have established a fully validated human BAC library covering the entire genome, enabling a fully autonomous workflow from sample preparation to probe development. Our BAC platform provides efficient and reliable solutions for basic research, disease mechanism studies, and molecular diagnostic development.
By determining the exact sequences at both ends of BAC inserts, our Sanger and Illumina-based platforms enable accurate clone localization, genome alignment, and physical map construction
02 BAC Library Screening Service
Accelerate your discovery process with Creative Biogene's fast and reliable BAC Library Screening service. Our optimized workflow ensures high sensitivity, short turnaround times, and seamless integration with downstream applications.
With extensive expertise in genetic engineering and proprietary technologies, Creative Biogene has developed a comprehensive BAC library construction and validation workflow, covering the entire process from sample preparation and DNA fragmentation to vector construction, clone screening, and library sequencing.
1. Sample Preparation and High-Molecular-Weight DNA Extraction
Optimized cell lysis and DNA extraction protocols ensure the integrity and purity of high-molecular-weight DNA, providing high-quality templates for BAC cloning. All procedures are performed under strict contamination-free conditions to guarantee library stability and reproducibility.
2. DNA Fragmentation and Vector Ligation
Genomic DNA is digested using rare-cutting restriction enzymes to generate fragments of approximately 150–200 kb, which are then inserted into BAC vectors via homology-based ligation. The ligated products are transformed into host bacteria to stably maintain large DNA fragments.
3. Library Amplification and Quality Verification
Each BAC clone undergoes growth selection, insert size verification, and random sequencing to ensure uniform coverage, minimal redundancy, and absence of large deletions. Creative Biogene's libraries achieve over 99% genome coverage, with a detailed clone ID and positional database for efficient retrieval and custom screening.
Fluorescence in situ hybridization (FISH) is widely used for chromosome structure analysis and gene localization. The specificity and signal intensity of FISH probes largely depend on the accuracy and integrity of the source material. BAC libraries serve as ideal templates for high-quality FISH probe preparation.
By leveraging Creative Biogene's BAC platform, we can rapidly and accurately develop FISH probes covering various chromosomal regions, suitable for research, clinical, and industrial applications.
FISH Probe Development Workflow with BAC Libraries:
BAC libraries are not only vital tools for genomics research but also critical enablers of modern molecular diagnostics, precision medicine, and structural biology. Leveraging whole-genome BAC resources, rigorous quality control, and professional customization, Creative Biogene delivers high-quality BAC library construction and application solutions to support precise and efficient gene research and clinical translation.
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