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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | AsPC-1 is a human pancreatic adenocarcinoma cell line originally derived from the ascites of a 62-year-old female patient with pancreatic cancer. This cell line is well-known for its high metastatic potential and ability to form mucinous tumors. AsPC-1 cells exhibit an epithelial cell morphology and possess several key gene alterations common in pancreatic cancer, including mutations in the KRAS and TP53 genes. This cell line is an important in vitro and in vivo model for studying the progression of pancreatic ductal adenocarcinoma (PDAC), chemotherapy resistance, and the development of novel targeted or immunotherapies. |
| Tissue | Pancreas; derived from metastatic site (Ascites) |
| Disease | Adenocarcinoma; Pancreatic Cancer |
| Morphology | Epithelial |
| Gender | Female |
| Age | 62 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Pancreatic cancer metastasis and invasion studies 2. Drug resistance evaluation and novel chemotherapeutic drugs 3. Mucin expression and its role in cancer biology 4. Xenograft and orthotopic animal model construction 5. KRAS-driven signaling pathways and their targeted inhibition studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Complex; Hypertriploid/Hypotetraploid |
| Tumorigenic | Yes, in nude mice |
| Antigen Expression | Blood group A; Rh-; HLA A2, B35, Bw52 |
| Genetic Profile | KRAS mutation; TP53 mutation; CDKN2A (p16) deletion |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all serum residue. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 15 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Aliquot the cell suspension into new culture containers. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00735 | GFP Reporter Cell Line - AsPC-1 | Inquiry |
| CSC-RR00736 | Luciferase Reporter Cell Line - AsPC-1 | Inquiry |
| CSC-RR01010 | GFP/Luc Reporter Cell Line - ASPC-1 | Inquiry |
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