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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-OV-3 is a human ovarian cancer cell line that was first isolated in 1973 from the malignant ascites of a 64-year-old Caucasian female with ovarian adenocarcinoma. SK-OV-3 cells are commonly used in cancer research to study ovarian cancer biology, drug resistance mechanisms, and explore potential therapeutic targets. The cell line is characterized by its epithelial morphology, high tumorigenicity, and expression of various ovarian cancer markers. |
| Tissue | Ovary |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 64 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Studying the biology and behavior of ovarian cancer cells 2. Drug screening and development 3. Genomic and proteomic analysis 4. 3D cell culture |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Early work by Fogh, J. in 1986 showed that SK-OV-3 cells did not express the MUC16 (CA125) mucin antigen (later the most commonly used biomarker for ovarian cancer detection), also showed using dose-response curves that SK-OV-3 were platinum sensitive. Subsequent studies showed that ectopic expression of the MUC16 C-terminal domain in SK-OV-3 cells reduced their sensitivity to cisplatin-induced apoptosis. |
| Characteristics | |
| Antigen Expression | Blood Type B; Rh+ |
| Tumorigenic | Yes, tumors form in nude mice. SK-OV-3 has been shown to produce large solid tumors (>1.5 cm^3) when injected into nude mice that are loosely adherent to the fat of the pelvic area, intestine, and/or omentum. |
| Mycoplasma Test | Negative |
| Cytogenetic Information | The SK-OV-3 cell line is also hypodiploid, with a chromosome mode of 43 (range 42–45) present in 63.3% of cells. |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 ml of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:6 is recommended. |
| Culture Medium | McCoy's 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO1107M | Cas9 Stable Cell Line - SKOV3 | Inquiry |
| CSC-RR0060 | GFP/Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR0061 | Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR0062 | RFP Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR01054 | mScarlet/LUC Reporter Cell Line - SK-OV-3 | Inquiry |
| CSC-RR0112 | GFP Stable Cell Line-SK-OV-3 | Inquiry |
| CSC-RT2603 | Human RIPK1 Knockout Cell Line-SK-OV-3 | Inquiry |
| CSC-RT2719 | Human MDM2 Knockout Cell Line - SK-OV-3 | Inquiry |
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