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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | CT-2A is a murine astrocytoma cell line originally induced by the intracranial implantation of 20-methylcholanthrene into C57BL/6 mice. It is one of the most widely utilized syngeneic models for the study of high-grade gliomas (glioblastomas). CT-2A cells exhibit a spindle-shaped or stellate morphology, characterized by highly aggressive growth, extensive vascularization, and a significant metabolic shift toward aerobic glycolysis (the Warburg effect). As this cell line is syngeneic with the C57BL/6 mouse strain, it serves as a preferred model for investigating the tumor immune microenvironment and cancer stem cell biology, as well as for evaluating metabolic inhibitors or immunotherapies within immunocompetent hosts. |
| Tissue | Brain |
| Disease | Glioblastoma (High-grade) |
| Morphology | Spindle-shaped; Pleomorphic |
| Gender | Not specified (derived from C57BL/6 mouse) |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Glioblastoma pathogenesis and malignant progression research 2. Study of tumor metabolism and the Warburg effect 3. Development of syngeneic orthotopic and subcutaneous brain tumor models 4. Evaluation of immune checkpoint inhibitors and dendritic cell vaccines 5. Investigation of glioma stem cells (GSCs) and radioresistance |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic C57BL/6 mice |
| Metabolic Profile | Highly glycolytic; low respiratory capacity |
| Genetic Profile | Maintains features of high-grade astrocytoma; highly invasive in vivo |
| Markers | Expresses nestin and other neural progenitor markers; GFAP expression varies |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically requiring 3 to 7 minutes). 4. Add complete growth medium to neutralize the trypsin, and gently pipette to create a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00726 | Luciferase Reporter Cell Line - CT-2A | Inquiry |
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