Saos-2 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	Saos-2 (osteosarcoma) is a cell line that was isolated by Fogh et al. in 1973 from a primary osteosarcoma in an 11-year-old Caucasian girl. Saos-2 cells express the 1,25-dihydroxyvitamin D3 receptor and have high basal alkaline phosphatase activity. They express the parathyroid hormone (PTH) receptor and produce cyclic adenosine monophosphate after PTH treatment. These cells do not form tumors when injected subcutaneously into immunocompromised mice. However, when injected into diffusion chambers implanted intraperitoneally in immunocompromised mice, Saos-2 cells produce mineralized matrix, a defining characteristic of osteoblasts. These cells have become a widely accepted model for studying osteosarcoma and bone biology due to their osteogenic properties and ability to produce a bone-like extracellular matrix. Saos-2 cells also exhibit an aneuploid karyotype, lacking several chromosomes but having extra copies of other chromosomes, which is typical of many cancer cell lines. They are negative for mycoplasma and have a robust calcification capacity, making them suitable for use in assays related to mineral deposition.
Tissue	Bone
Disease	Osteosarcoma
Morphology	Epithelial
Gender	Female
Age	11 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug development and screening 3. Bone formation and osteogenesis 4. Mineralization studies 5. Cell signaling pathways 6. Biomaterials testing
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Some advantages of using the Saos-2 cell line include that they have well-documented characterization data, large numbers of cells can be obtained in a short period of time, and that Saos-2 cells can be fully differentiated in the same way that osteoblasts naturally differentiate.
Characteristics
Karyotype	2n = 46, (P1) Hyperploid to hypopentaploid
Receptors Expressed	Epidermal growth factor (EGF), transforming growth factor beta (type 1 and type 2)
Antigen Expression	Blood Type B, Rh+, HLA A2, A3, Bw16, Bw47
Tumorigenic	No, Saos-2 cells were not tumorigenic in immunosuppressed mice but formed colonies in semisolid culture medium.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:2 to 1:4 is recommended.
Culture Medium	McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

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* For research use only. Not intended for any clinical use.

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