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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The ZJU-0430 gallbladder cancer (GBC) cell line was derived from a 74-year-old male patient who presented with abnormal serum ferritin levels and whose gallbladder cancer was pathologically classified as a well-differentiated adenocarcinoma. Under microscopic examination, this cell line exhibits diverse cellular morphologies and typical epithelial cell characteristics; however, scRNA-Seq analysis revealed that its internal heterogeneity is not significant. Its population doubling time (PDT) is 19.81 hours, which is shorter than that of the GBC-SD cell line. Immunophenotypic analysis results indicate that ZJU-0430 cells highly express CD24, CD44, CD29, and CD133; partially express CD184 and CD326; and do not express CD34, CD90, CD117, or CD338. Compared to GBC-SD cells, ZJU-0430 cells demonstrate superior migratory, invasive, and proliferative capabilities. Furthermore, in a nude mouse xenograft model, these cells also exhibited in vivo tumorigenicity. Consequently, the ZJU-0430 cell line serves as a highly representative model for the disease and is suitable for preclinical studies investigating the pathogenesis and therapeutic strategies of gallbladder cancer. |
| Tissue | Gallbladder |
| Disease | Gallbladder Adenocarcinoma |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | 74 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Gallbladder cancer (GBC) pathogenesis and molecular biology research 2. Screening for novel chemotherapeutic and targeted anti-tumor agents 3. Investigation of gallbladder tumor cell migration and epithelial-mesenchymal transition (EMT) 4. Study of the biliary tract cancer genomics and signaling pathways 5. Development of human tumor xenograft models in immunocompromised mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | Exhibits genomic instability; often used to study biliary-specific driver mutations |
| Phenotype | Maintains high-grade malignant features of biliary adenocarcinoma |
| Growth Kinetics | Stable growth in standard culture media; strong adherence |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| CSC-RR00862 | Luciferase Reporter Cell Line - ZJU-0430 | Inquiry |
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