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RNA-seq Profiling Services


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RNA-seq Profiling Services

Recently, researchers have begun to put together a more comprehensive picture surrounding the “central dogma” of molecular biology, leading to the revelation that RNA is not just a simple genetic messenger, but rather a complex signaling molecule which is present in an increasing number of structural forms. A variety of iterations in which RNA exists allow the molecule to function as the template for translating the genetic code into protein, as the post-transcriptional regulator and the gene silencer, additionally, as recently discovered, a modulator of epigenetic elements.

Specific classes of non-coding RNA (ncRNA) ―short RNAs (including micro RNA (miRNA), short interfering RNA (siRNA), and piwi-interacting RNA (piRNA)) and long non-coding RNAs (lncRNA)―regulate gene expression through epigenetic mechanisms, influencing several cellular processes, such as X chromosome inactivation, genomic imprinting, some cellular processes, and cancer. LncRNAs tether epigenetic complexes that are capable of methylating DNA and modifying histones to the chromatin, thereby enabling allele and locus-specific regulation. Moreover, lncRNAs, because of their length, are suited for orchestrating specific regulatory events particular to a target locus. In addition, miRNA has shown to be linked with cancer and can act as oncogenes or tumor-suppressor genes. They can be involved in establishing DNA methylation and regulating histone modification through interacting with their target mRNA to alter chromatin of the corresponding DNA template.

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Figure 1. The function of ncRNA in living organisms.

With the development of next-generation sequencing applied to RNA, also known as RNA-Seq, researchers have been able to create accurate and quantifiable data for ncRNA regulation mechanisms. Furthermore, RNA-Seq is positioning itself as a truly translational research tool, seeking to improve current diagnostic techniques and even displace traditional gold-standard clinical methodologies.

Creative Biogene, as a leading biotechnology company, is able to offer a comprehensive range of RNA-seq profiling services to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome.

Bioinformatics AnalysisSpecific Description
mRNA analysis
  • Gene expression profiling and differentially expressed genes (DEG) identification
  • Protein-coding RNA with specific poly A selection
  • Aberrant mRNA translation in cancer pathogenesis
  • Cancer-related mRNA signatures
  • The epigenetic mechanism of gene expression
Small ncRNA analysis
  • Differential miRNA expression
  • Post-transcriptional regulation of gene expression
  • Novel transcripts and differentially-expressed small RNAs
  • miRNAs involved in regulation of onco- and tumor-suppressor gene expression
LncRNA analysis
  • Non-protein coding RNAs involved in chromatin remodeling and transcriptional/post-transcriptional regulation
Transcriptome sequencing
  • Analysis of the whole transcriptome
  • Analysis of RNAs larger than 200nt with optional rRNA depletion

Features

  • Superior data quality
  • Flexible extraction and data analysis options available
  • Complete sample-to-bioinformatics solution
  • Dedicated support and industry-leading turnaround time

Creative Biogene provides a comprehensive RNA-Seq service, including everything from the isolation of RNA to data analysis. Combine our worldwide leading ChIP-Seq services with RNA-Seq technology to gain an even greater understanding of epigenetic mechanisms. Our highly experienced scientists will also consult with you on project-specific details and outline an appropriate experimental design. In addition, custom bioinformatics and data analysis services are also available.

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