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Recently, researchers have begun to put together a more comprehensive picture surrounding the “central dogma” of molecular biology, leading to the revelation that RNA is not just a simple genetic messenger, but rather a complex signaling molecule which is present in an increasing number of structural forms. A variety of iterations in which RNA exists allow the molecule to function as the template for translating the genetic code into protein, as the post-transcriptional regulator and the gene silencer, additionally, as recently discovered, a modulator of epigenetic elements.
Specific classes of non-coding RNA (ncRNA) ―short RNAs (including micro RNA (miRNA), short interfering RNA (siRNA), and piwi-interacting RNA (piRNA)) and long non-coding RNAs (lncRNA)―regulate gene expression through epigenetic mechanisms, influencing several cellular processes, such as X chromosome inactivation, genomic imprinting, some cellular processes, and cancer. LncRNAs tether epigenetic complexes that are capable of methylating DNA and modifying histones to the chromatin, thereby enabling allele and locus-specific regulation. Moreover, lncRNAs, because of their length, are suited for orchestrating specific regulatory events particular to a target locus. In addition, miRNA has shown to be linked with cancer and can act as oncogenes or tumor-suppressor genes. They can be involved in establishing DNA methylation and regulating histone modification through interacting with their target mRNA to alter chromatin of the corresponding DNA template.
Figure 1. The function of ncRNA in living organisms.
With the development of next-generation sequencing applied to RNA, also known as RNA-Seq, researchers have been able to create accurate and quantifiable data for ncRNA regulation mechanisms. Furthermore, RNA-Seq is positioning itself as a truly translational research tool, seeking to improve current diagnostic techniques and even displace traditional gold-standard clinical methodologies.
Creative Biogene, as a leading biotechnology company, is able to offer a comprehensive range of RNA-seq profiling services to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome.
|Bioinformatics Analysis||Specific Description|
|Small ncRNA analysis|
Creative Biogene provides a comprehensive RNA-Seq service, including everything from the isolation of RNA to data analysis. Combine our worldwide leading ChIP-Seq services with RNA-Seq technology to gain an even greater understanding of epigenetic mechanisms. Our highly experienced scientists will also consult with you on project-specific details and outline an appropriate experimental design. In addition, custom bioinformatics and data analysis services are also available.