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Cell-free Expression System

Creative Biogene is a leading biotechnology company which understands the importance of convenient and easy-to-use systems for the expression of proteins including toxic proteins, large proteins and membrane proteins that are challenging for traditional cell-based expression systems. With years of development, Creative Biogene has developed cell-free expression systems to meet the increasing demands for these particular protein types. Creative Biogene is confident to provide you with the most affordable and high-quality gene expression services using cell-free expression systems to ensure your satisfaction in a timely and professional manner.

Cell-free expression system is a system that enables researchers to rapidly express and manufacture small amount of functional proteins in vitro through translation machineries extracted from cells. Generally, components of a cell free reaction include a cell extract, an energy source, a supply of amino acids, cofactors and the gene of your interest. Cell-free expression system overcomes many obstacles of conventional cellular expression. It is a preferable choice for many applications in protein research including options for protein labeling and the expression of difficult-to-express proteins such as toxic proteins, membrane proteins and multiple protein complexes. Creative Biogene which possesses advanced technologies and expertise experience can offer a wide variety of cell-free expression systems. Creative Biogene’s goal is to provide you with the most affordable, high-quality gene expression services through cell-free expression system in a short turnaround time.

A schematic overview of the cell-free protein production process [1]


  • Expression of difficult to express proteins, e.g. membrane proteins, transcription factors, toxic proteins
  • Studies on protein-protein, protein-DNA, or protein-RNA interactions
  • Functional analysis of mutations, truncations, protein domains
  • Protein engineering
  • Protein labeling with stable isotopes


  • One-stop solution from gene synthesis/gene cloning to purified protein
  • Scalable protein production and automation (µg to g amounts)
  • Simultaneous expression of proteins in one reaction
  • Professional support: our experienced scientist will serve as your personal consultant from design to delivery
  • Short timeline from gene synthesis/cloning to protein recovery
  • Cost-saving: provide high quality proteins with competitive price

Creative Biogene provides gene expression service through cell-free expression system, which includes:

  • Codon optimization for any given expression system (upon request)
  • DNA template preparation for in vitro transcription
  • Small-scale screening for protein translation efficiency
  • Scale-up of protein production and purification
  • Protein post processing (optional) such as purified protein tag removal, filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing et al.)


  1. Krinsky N., et al. (2016). A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis. PloS one,11 (10), e0165137.
  2. Roos C., et al. (2014). High-level Cell-free production of membrane proteins with Nanodiscs. Cell-Free Protein Synthesis: Methods and Protocols, 109-130.
  3. Imataka H. & Mikami S. (2009). Advantages of human cell-derived cell-free protein synthesis systems. Seikagaku. The Journal of Japanese Biochemical Society, 81(4), 303-307.

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