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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | TE-1 is a mature human cancer cell line derived from well-differentiated esophageal squamous cell carcinoma. Originally isolated at Tohoku University in Japan in the 1970s, this cell line is characterized by its epithelial morphology and ability to grow in vitro as single cells or cell stacks. Biologically, TE-1 cells are crucial for maintaining key structural features of the esophageal epithelium, such as desmosomes and finger-like microvilli. They are widely used in studies of the molecular pathogenesis of esophageal cancer and have been shown to form tumors in immunodeficient mice that are highly histologically similar to those of primary patient tumors. |
| Tissue | Esophagus |
| Disease | Esophageal Squamous Cell Carcinoma (ESCC) |
| Morphology | Epithelial-like; Polygonal |
| Gender | Male |
| Age | 58 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 |
| Applications | 1. Esophageal Cancer Research 2. Drug Discovery and Chemosensitivity 3. Receptor Signaling Assays 4. Toxicology and Carcinogenesis 5. High-Throughput Genomic Screening |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Hypotriploid/Hyperdiploid; modal chromosome number typically around 52; contains distinctive marker chromosomes. |
| Tumorigenic | Yes, highly tumorigenic in nude or SCID mice. |
| Genome Variants | Associated with p53 signaling pathway alterations and dysregulation of cell cycle regulatory genes. |
| Markers Expressed | Positive for epithelial markers; demonstrates abundant organelles (mitochondria and RER) and desmosomes. |
| Receptors Expressed | High affinity epidermal growth factor receptors (EGFR). |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks). 4. Observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). Do not tap or shake the culture flask to avoid cell clumping. 5. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 6. Centrifuge the cell suspension at 125 x g for 5 to 7 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | 2 to 3 times per week. |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended for routine maintenance. |
| Culture Medium | RPMI-1640 Medium + 10% Fetal Bovine Serum (FBS). Some formulations may include 2 mM L-glutamine and 4500 mg/L glucose. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 90% to 95% complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02559 | Human CHST1 Stable Cell Line - TE-1 | Inquiry |
| CSC-RR00847 | Luciferase Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01083 | mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01084 | AP1-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01085 | ARE-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01086 | ISRE-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01087 | Myc-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01088 | NFκB-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01089 | p53-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01090 | STAT3-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01091 | IL6pro-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
| CSC-RR01092 | TNFαPro-Luc/EF1α-mCherry Reporter Cell Line - TE-1 | Inquiry |
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