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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | 1321N1 is a human astrocytoma cell line isolated in 1972, a subclone of the 1181N1 cell line (originally derived from U-118 MG). Studies have shown that its ancestral lineage is related to the U-138 MG astrocytoma cell line. These cells exhibit a polygonal morphology and typical glial cell characteristics. They hold significant biological importance due to their expression of multiple muscarinic receptors (M2, M3, and M5) and β2-adrenergic receptors, participating in the phosphatidylinositol signaling pathway. Upon activation, these cells release IP3, triggering intracellular calcium waves and inducing ATP release, making them an ideal model for studying intercellular communication in the central nervous system. |
| Tissue | Brain |
| Disease | Astrocytoma |
| Morphology | Glial; Polygonal |
| Gender | Male |
| Age | 47 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Neuropharmacology and Drug Screening 2. Receptor Signaling Research 3. Cancer Biology and Glioma Studies 4. Genetic Manipulation and Reporter Assays |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Hypodiploid; contains chromosomal abnormalities |
| Mutations | Homozygous TP53 mutation (p. Arg213Gln); PTEN splice site mutation (c. 1026+1G>T); mitochondrial DNA mutations in complexes I and IV |
| Receptors Expressed | M2, M3, and M5 muscarinic receptors; β2-adrenergic receptors (β2-AR) |
| Markers Expressed | GFAP, Vimentin, S-100 protein |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks) and observe under an inverted microscope until the cell layer disperses (usually 2 to 10 minutes). 4. Add an equal volume of complete culture medium to neutralize the trypsin. 5. Gently pipette the cells and transfer them to centrifuge tubes. 6. Centrifuge at 125 x g for 5-7 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | A split ratio of 1:2 to 1:6 is recommended. |
| Culture Medium | DMEM (Dulbecco's Modified Eagle Medium) + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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