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HT29 Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionHT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. HT-29 is sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which is a standard treatment option for colorectal cancer. In addition to being a xenograft tumor model for colorectal cancer, the HT-29 cell line is also used as an in vitro model to study absorption, transport, and secretion by intestinal cells. Under standard culture conditions, these cells grow as an undifferentiated and nonpolarized multilayer. However, altering culture conditions or treating the cells with various inducers can result in a differentiated and polarized morphology, characterized by the redistribution of membrane antigens and development of an apical brush-border membrane.
DiseaseColorectal adenocarcinoma
Product FormatFrozen
Growth ModeAdherent
Age44 years adult
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsHT29 cell line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Lactobacillus and Bifidobacterium strains to counteract the toxic effect of C. difficile LMG21717.
Shipped inDry ice
Storage Temperature−196°C
Karyotype2n = 46, hypertriploid
Antigen ExpressionBlood Type A; Rh+; A3, B12, B17, Cw5, HLA A1
OncogeneMyc +; abl -; ras +; myb +; fos +; sis +; p53 +; ros -; src -
Cellular ProductsSecretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin
SterilityBacteria: Negative; Yeast: Negative; Mycoplasma: Negative
PathogensHepatitis B: Negative; Hepatitis C: Negative; HIV: Negative
EffectsYes, in nude mice; forms well-differentiated adenocarcinoma consistent with colonic primary; tumors also form in steroid treated hamsters.
Culture Conditions and Handling
Culture MediumMcCoy’s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.
Subculturing1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask; observe cells under an inverted microscope until cell layer is dispersed.
4. Add 6.0 to 8.0 mL of complete growth medium, aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Split RatioA subcultivation ratio of 1:3 to 1:8 is recommended.
Medium Renewal2 to 3 times per week
Incubation ConditionCarbon dioxide (CO2), 5%; 37°C
Freeze MediumComplete culture medium supplemented with 5% (v/v) DMSO
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CSC-RR0451Luciferase Stable Cell Line - HT29Inquiry

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For research use only. Not intended for any clinical use.

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