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|Organism||Homo sapiens, human|
|Cell Line Description||HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. HT-29 is sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which is a standard treatment option for colorectal cancer. In addition to being a xenograft tumor model for colorectal cancer, the HT-29 cell line is also used as an in vitro model to study absorption, transport, and secretion by intestinal cells. Under standard culture conditions, these cells grow as an undifferentiated and nonpolarized multilayer. However, altering culture conditions or treating the cells with various inducers can result in a differentiated and polarized morphology, characterized by the redistribution of membrane antigens and development of an apical brush-border membrane.|
|Age||44 years adult|
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
|Applications||HT29 cell line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Lactobacillus and Bifidobacterium strains to counteract the toxic effect of C. difficile LMG21717.|
|Shipped in||Dry ice|
|Karyotype||2n = 46, hypertriploid|
|Antigen Expression||Blood Type A; Rh+; A3, B12, B17, Cw5, HLA A1|
|Oncogene||Myc +; abl -; ras +; myb +; fos +; sis +; p53 +; ros -; src -|
|Cellular Products||Secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin|
|Sterility||Bacteria: Negative; Yeast: Negative; Mycoplasma: Negative|
|Pathogens||Hepatitis B: Negative; Hepatitis C: Negative; HIV: Negative|
|Effects||Yes, in nude mice; forms well-differentiated adenocarcinoma consistent with colonic primary; tumors also form in steroid treated hamsters.|
|Culture Conditions and Handling|
|Culture Medium||McCoy’s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.|
|Subculturing||1. Remove and discard culture medium.|
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask; observe cells under an inverted microscope until cell layer is dispersed.
4. Add 6.0 to 8.0 mL of complete growth medium, aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
|Split Ratio||A subcultivation ratio of 1:3 to 1:8 is recommended.|
|Medium Renewal||2 to 3 times per week|
|Incubation Condition||Carbon dioxide (CO2), 5%; 37°C|
|Freeze Medium||Complete culture medium supplemented with 5% (v/v) DMSO|
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.