Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Canis familiaris, dog |
| Cell Line Description | Madin-Darby Canine Kidney (MDCK) cells are widely used as models for studying epithelia as they have clear apico-basolateral polarity, well defined cell junctions, a rapid growth rate, are suitable for confocal imaging and will polarise in 2D and 3D cell culture. Two additional subtypes of MDCK cells were isolated from the parental strain and named type I and type II MDCK cells. MDCK I cells were isolated from low-passage parental MDCK cells and displayed very high transepithelial resistance (TER) values (>4000 Ω·cm2), indicating very "tight" connections. MDCK II cells were obtained from higher passage MDCK cells and displayed much lower TER values (< 300 Ω·cm2), indicating "leaky" junctions. This difference in TER results from differences in the composition of its tight junctions. Both strains express the tight junction proteins claudin-1, claudin-4, occludin, and ZO-1. However, type II cells also express the pore-forming tight junction protein claudin-2, which may reduce TER in MDCK II cells. |
| Tissue | Kidney |
| Morphology | Epithelial-like |
| Disease | Normal |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Drug permeability studies 2. Virology studies 3. Toxicology studies 4. Cell biology studies 5. Gene expression Studies 6.. Oncology studies |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Although MDCK I cells stained for E-cadherin much more strongly than MDCK II cells, both strains formed adherens junctions and desmosomes. In contrast, MDCK II cells showed stronger basal desmosome staining. Finally, there are differences in the formation of gap junctions. Type I cells have gap junctions, whereas type II cells do not form gap junctions unless gap junction formation is forced by the expression of gap junction proteins such as connexin 43. In addition to differences in junctions, these strains also differ in size, with MDCK II cells being larger and taller compared to smaller, flattened type I cells. |
| Characteristics | |
| Genes Expressed | claudin-1, claudin-2, claudin-4, occludin, and ZO-1 |
| Virus Susceptibility | Influenza virus, vesicular stomatitis virus and many other viruses. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:6 is recommended. |
| Culture Medium | MEM + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0680 | Human ABCB1 Stable Cell Line - MDCK-II | Inquiry |
| CSC-RO0907 | Human B2M/FCGRT Stable Cell Line - MDCK-II | Inquiry |
| CSC-RT2797 | STAT1 Knockout Cell Line-MDCKII | Inquiry |
| CSC-SC000069-1 | Human ABCG2 Stable Cell Line - MDCK-II | Inquiry |
Copyright © Creative Biogene. All rights reserved.