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RNA interference (RNAi), which reacts to the presence of double-stranded RNA, is a conserved biological response. It provides resistance to both inherent parasitic and external pathogenic nucleic acids, as well as controls the expression of protein-coding genes. Its properties have enabled researchers to exploit RNAi as a tool for selective gene function study. With the introduction of genome-wide or gene set libraries of small interfering RNAs (siRNAs) targeting variant organisms, its use can now be truly systematized.
Creative Biogene, a global leader in biotechnology, offers a range of siRNA libraries, including but not limited to Human Genome siRNA Library, Human Kinase siRNA Library, and Human GPCR siRNA Library. The overarching aim is to offer superior products to expedite scientific investigations into uncovering unidentified elements of biological pathways or therapeutic targets.
Key Features of Our siRNA Libraries
RNA interference (RNAi) is a universally preserved biological response to the presence of double-stranded RNA, serving to protect against both intrinsic parasitic and extrinsic pathogenic nucleic acids, while also controlling the expression of protein-encoding genes. With these unique properties, scientists have utilized RNAi as an instrument to selectively explore gene functionality, and the introduction of genome-wide or subsets of small interfering RNAs (siRNAs) libraries that target a wide variety of organisms, this has allowed for its usage in a systemically pertinent manner. Our products can help you in the following aspects:
Case Study 1
Andes virus (ANDV) stands as the primary culprit behind hantavirus pulmonary syndrome (HPS) in South America, where its high fatality rate (up to 40%) remains unaddressed by approved vaccines or antiviral treatments. Researchers employed a comprehensive approach, utilizing an siRNA library targeting 140 membrane trafficking-related genes, to investigate ANDV infection in human lung microvascular endothelial cells. Through this screening, 55 essential genes were identified, influencing major endocytic pathways, endosomal transport, cell signaling, and cytoskeleton rearrangement. Subsequent analysis, involving infectious ANDV and retroviral pseudovirions, further characterized the involvement of 9 selected genes in early ANDV entry steps. Additionally, the study utilized cellular endocytosis markers and chemical inhibitors, revealing that ANDV exploits multiple entry routes, predominantly clathrin-, dynamin-, and cholesterol-dependent, with potential clathrin-independent mechanisms.
Figure 1. Researchers utilized an siRNA library (siRNA pools against ANDV N segment) to identify genes in Andes virus infection of primary human lung cells (HMVEC-L). The results, presented as percentages of ANDV-infected cells after siRNA administration, were compared with a negative control set, emphasizing the comprehensive approach employed in the study. (Chiang CF, et al., 2016)
Case Study 2
Researchers employed a 4 x 106 siRNA library to uncover mechanisms of epirubicin resistance in gastric cancer. Two identified siRNAs targeting GAS1 and PTEN revealed GAS1 suppression led to significant epirubicin resistance, cross-resistance to 5-fluorouracil and cisplatin, and induced multidrug resistance by inhibiting apoptosis. GAS1 suppression increased drug efflux through P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP). Targeted knockdown of P-gp and BCRP partially reversed GAS1 suppression-induced resistance. These findings position GAS1 as a potential target for overcoming multidrug resistance in gastric cancer.
Figure 2. Researchers performed sequence analysis and validated the effectiveness of GAS1 and PTEN siRNAs obtained from a random siRNA library. The inhibition of GAS1 and PTEN expression in SGC7901 cells was confirmed through transfection with psiRNA-lib-GAS1, psiRNA-lib-GAS1(+), psiRNA-lib-PTEN, and psiRNA-lib-PTEN(+). (Zhao L, et al., 2009)
Q: How do siRNA libraries differ from single siRNA molecules in terms of their utility in gene function studies?
A: siRNA libraries offer a comprehensive collection of siRNA molecules targeting various genes, enabling systematic and high-throughput gene function studies across entire genomes or specific gene sets. In contrast, single siRNA molecules target individual genes and are more suitable for targeted knockdown experiments on specific genes of interest.
Q: What advantages do Creative Biogene's siRNA libraries offer for drug target discovery compared to traditional methods?
A: Creative Biogene's siRNA libraries, such as the Human Druggable Genome siRNA Library, provide a systematic approach for identifying potential drug targets by targeting a wide range of genes involved in drug metabolism, signaling pathways, and disease mechanisms. This comprehensive coverage accelerates the drug discovery process and facilitates the optimization of therapeutic strategies.
Q: How can the Human Kinase siRNA Library contribute to the study of cellular signaling pathways?
A: The Human Kinase siRNA Library allows researchers to systematically investigate the regulatory roles of protein kinases in cellular signaling pathways. By silencing kinase genes using siRNA molecules from the library, researchers can elucidate the functions of specific kinases and their downstream signaling cascades, advancing our understanding of cell signaling mechanisms.
Q: What measures are taken to ensure the quality and specificity of siRNA molecules in Creative Biogene's siRNA libraries?
A: Creative Biogene's siRNA libraries undergo rigorous quality control measures, including sequence verification and validation, to ensure the accuracy and specificity of the siRNA molecules. This verification process ensures that the siRNA sequences accurately target the intended genes, minimizing off-target effects and ensuring reliable results in gene knockdown experiments.
| Cat.No. | Product Name | Price |
|---|---|---|
| HSLR-01 | Human Genome siRNA Library | Inquiry |
| HSLR-02 | Human Druggable Genome siRNA Library (96-well) | Inquiry |
| HSLR-03 | Human Druggable Genome siRNA Library (384-well) | Inquiry |
| HSLR-04 | Human Kinase siRNA Library | Inquiry |
| HSLR-05 | Human Phosphatase siRNA Library | Inquiry |
| HSLR-06 | Human Protease siRNA Library | Inquiry |
| HSLR-07 | Human GPCR siRNA Library | Inquiry |
| HSLR-08 | Human Nuclear Hormone siRNA Library | Inquiry |
| HSLR-09 | Human Ion Channel siRNA Library | Inquiry |
| HSLR-10 | Human Epigenetics siRNA Library | Inquiry |
| HSLR-11 | Human Transcription Factor siRNA Library | Inquiry |
| HSLR-12 | Human DNA Damage Response siRNA Library | Inquiry |
| HSLR-13 | Human Drug Targets siRNA Library (96-well) | Inquiry |
| HSLR-14 | Human Drug Targets siRNA Library (384-well) | Inquiry |
| HSLR-15 | Human Apoptosis siRNA Library | Inquiry |
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