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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H838 (also known as H838) is an epithelial-morphological human non-small cell lung cancer (NSCLC) cell line. Established in 1984, it originated from the lymph nodes of a 59-year-old Caucasian male patient with stage IV lung adenocarcinoma. This patient had a smoking history (100 pack-years). NCI-H838 cells are widely used in lung oncology to study the molecular drivers of lung adenocarcinoma and to test the efficacy of targeted therapy and immunotherapy. This cell line is characterized by high expression of epidermal growth factor receptor (EGFR) and wild-type responses to several common lung cancer mutations, making it an important control or comparative model in genomic studies. |
| Tissue | Lung; derived from metastatic site (Lymph node) |
| Disease | Adenocarcinoma; Non-Small Cell Lung Cancer (NSCLC) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 59 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Lung cancer biology and adenocarcinoma progression research 2. Screening for novel targeted therapies and chemotherapeutic agents 3. Study of EGFR signaling pathways and protein expression 4. Investigation of tumor metabolism and oxidative stress response 5. Development of human tumor xenograft models in immunocompromised mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in nude mice |
| Karyotype | Aneuploid; highly rearranged chromosomal structure |
| Genetic Profile | TP53 mutation (deletion); KRAS wild-type; EGFR wild-type (high expression); CDKN2A (p16) homozygous deletion |
| Expression Markers | Positive for epithelial markers (e.g., Cytokeratins) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until the cell suspension is a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Medium | RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00810 | GFP Reporter Cell Line - NCI-H838 | Inquiry |
| CSC-RR00811 | Luciferase Reporter Cell Line - NCI-H838 | Inquiry |
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