Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Farage is a B lymphocyte cell line isolated in 1990 from a Caucasian adult female patient with non-Hodgkin B cell lymphoma. The cells lack both T and myeloid surface markers, express B cell surface antigens including CD19, CD20, CD22, HLA-DR, were positive for C3 receptors and EBNA and expressed BCL-2. No immunoglobulin determinants could be demonstrated on the cell surface. When treated with interleukin 4 (IL-4), Farage cells exhibit increased expression of several markers including CD23, CD54, and CD58, along with decreased levels of CD21, CD22, and CD38. This modulation of surface markers suggests a role for IL-4 in influencing B cell behavior and provides a useful model for exploring signaling pathways and regulatory mechanisms in B cells. The response to phorbol 12-myristate 13-acetate (PMA) treatment, which results in the down-regulation of CD21 and CD23, further supports its application in studying kinase-driven signaling in B-cells. Additionally, the presence of Epstein-Barr virus (EBV) in these cells allows for the study of viral interactions with host cell machinery, particularly in the context of lymphocyte oncogenesis. |
| Disease | Lymphoma; Non-Hodgkin's B Cell |
| Morphology | Lymphoblast |
| Gender | Female |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Development and Screening 3. Gene Expression Studies 4. Immunology Studies 5. Biological Pathway Analysis |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The Farage cells were negative for B cell activation antigens including CD25, CD11b, HC2 and Bly-7. They were strongly positive for both nuclear and cytoplasmic TdT. In addition the TCRγ gene but not the TCRβ was rearranged in one allele and the TCRα was not detected. |
| Characteristics | |
| Karyotype | Trisomy of chromosome 11 |
| Antigen Expression | CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR + |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Cultures can be maintained by adding fresh medium or changing the medium. Alternatively, cultures can be established by centrifugation and resuspending at 3 - 5 x 105 viable cells/mL. Maintain cell density between 3 x 105 and 3 x 106 viable cells/mL. |
| Medium Renewal | Add fresh medium every 2 to 3 days. |
| Subcultivation Ratio | The ratio of 1:2 to 1:5 is recommended. |
| Culture Medium | 90% RPMI 1640 + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00610 | Luciferase Reporter Cell Line - Farage | Inquiry |
| CSC-RR00611 | GFP Reporter Cell Line - Farage | Inquiry |
| CSC-RR00620 | GFP/Luc Reporter Cell Line - Farage | Inquiry |
Copyright © Creative Biogene. All rights reserved.