B16F10 Cell Line
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B16F10 Cell Line
| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | B16F10 is a murine melanoma cell line from the C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice. |
| Strain | C57BL/6J |
| Tissue | Skin |
| Disease | Melanoma |
| Morphology | Mixture of spindle-shaped and epithelial-like cells |
| Gender | Unknown |
| Primary | No |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | Cell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| References | J. Natl. Cancer Inst. 60: 1217-1222, 1978 |
| Characteristics | |
| Images |  |
| Isoenzyme Analysis | NP, G6PD, MD, MPI, AST, LD and Pep B |
| Mycoplasma Test | Negative |
| Tumorigenic | Yes, in syngeneic mice |
| Comments | Useful for metastasis study. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| Culture Conditions and Handling | |
| Thawing Frozen Cells |
|
| Culture Medium | 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum. |
| Subculturing |
|
| Subcultivation Ratio | The ratio of 1:10 is recommended. |
| Medium Renewal | Every 2 to 3 days |
| Freeze Medium | 93% culture medium + 7% DMSO> |
| Culture Temperature | 37°C |
| Incubation Condition | 5% CO2 |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0231 | Luc/GFP Reporter Cell Line-B16F10 | Inquriy |
| CSC-RR0232 | Luc/RFP Reporter Cell Line-B16F10 | Inquriy |
| CSC-RR0233 | mSlc5a5/Luc Reporter Cell Line-B16F10 | Inquriy |
| CSC-RR0234 | Luc Reporter Cell Line-B16F10 | Inquriy |
| CSC-RR0235 | mSlc5a5/GFP Reporter Cell Line-B16F10 | Inquriy |
| CSC-RR0236 | mSlc5a5/RFP Reporter Cell Line-B16F10 | Inquriy |
| CSC-RO0016 | mSlc5a5 Overexpression Stable Cell Line-B16F10 | Inquriy |
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