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B16F10 Cell Line

General Information
OrganismMus musculus, mouse
Cell Line DescriptionB16F10 is a murine melanoma cell line from the C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice.
MorphologyMixture of spindle-shaped and epithelial-like cells
Product FormatFrozen
Growth ModeAdherent
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsCell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection
Shipped inDry ice
Storage Temperature−196°C
ReferencesJ. Natl. Cancer Inst. 60: 1217-1222, 1978
ImagesB16F10 Cell Line
Isoenzyme AnalysisNP, G6PD, MD, MPI, AST, LD and Pep B
Mycoplasma TestNegative
TumorigenicYes, in syngeneic mice
CommentsUseful for metastasis study. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210.
Culture Conditions and Handling
Thawing Frozen Cells
  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the oring and cap out of the water. Thawing should be rapid (about 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. For cells that are sensitive to DMSO is recommended that the cryoprotective agent be removed immediately. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5-7 minutes.
  4. Discard the supernatant and resuspend cell pellet with the complete medium.
  5. Incubate the culture in an appropriate atmosphere and temperature.
Culture Medium90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum.
  1. Remove medium, and rinse with PBS without calcium and magnesium.
  2. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
  3. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation RatioThe ratio of 1:10 is recommended.
Medium RenewalEvery 2 to 3 days
Freeze Medium93% culture medium + 7% DMSO>
Culture Temperature37°C
Incubation Condition5% CO2

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For research use only. Not intended for any clinical use.

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