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B16F10 Cell Line
| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | B16F10 is a murine melanoma cell line from the C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice. |
| Strain | C57BL/6J |
| Tissue | Skin |
| Disease | Melanoma |
| Morphology | Mixture of spindle-shaped and epithelial-like cells |
| Gender | Unknown |
| Primary | No |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | Cell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| References | J. Natl. Cancer Inst. 60: 1217-1222, 1978 |
| Characteristics | |
| Images |  |
| Isoenzyme Analysis | NP, G6PD, MD, MPI, AST, LD and Pep B |
| Mycoplasma Test | Negative |
| Tumorigenic | Yes, in syngeneic mice |
| Comments | Useful for metastasis study. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
| Culture Conditions and Handling | |
| Thawing Frozen Cells |
|
| Culture Medium | 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum. |
| Subculturing |
|
| Subcultivation Ratio | The ratio of 1:10 is recommended. |
| Medium Renewal | Every 2 to 3 days |
| Freeze Medium | 93% culture medium + 7% DMSO> |
| Culture Temperature | 37°C |
| Incubation Condition | 5% CO2 |
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| CSC-RR0234 | Luc Reporter Cell Line-B16F10 | Inquiry |
| CSC-RR0235 | mSlc5a5/GFP Reporter Cell Line-B16F10 | Inquiry |
| CSC-RR0236 | mSlc5a5/RFP Reporter Cell Line-B16F10 | Inquiry |
| CSC-RO0016 | mSlc5a5 Overexpression Stable Cell Line-B16F10 | Inquiry |
| CSC-RR0528 | ZsYellow1 Reporter Cell Line - B16F10 | Inquiry |
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
For research use only. Not intended for any clinical use.