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General Information | |
Organism | Mus musculus, mouse |
Cell Line Description | B16F10 is a murine melanoma cell line from the C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice. |
Strain | C57BL/6J |
Tissue | Skin |
Disease | Melanoma |
Morphology | Mixture of spindle-shaped and epithelial-like cells |
Gender | Unknown |
Primary | No |
Product Format | Frozen |
Growth Mode | Adherent |
Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications | Cell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection |
Shipped in | Dry ice |
Storage Temperature | −196°C |
References | J. Natl. Cancer Inst. 60: 1217-1222, 1978 |
Characteristics | |
Images | ![]() |
Isoenzyme Analysis | NP, G6PD, MD, MPI, AST, LD and Pep B |
Mycoplasma Test | Negative |
Tumorigenic | Yes, in syngeneic mice |
Comments | Useful for metastasis study. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cyclin and MC210. |
Culture Conditions and Handling | |
Thawing Frozen Cells |
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Culture Medium | 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum. |
Subculturing |
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Subcultivation Ratio | The ratio of 1:10 is recommended. |
Medium Renewal | Every 2 to 3 days |
Freeze Medium | 93% culture medium + 7% DMSO> |
Culture Temperature | 37°C |
Incubation Condition | 5% CO2 |
Cat.No. | Product Name | Price |
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CSC-RR0231 | Luc/GFP Reporter Cell Line-B16F10 | Inquiry |
CSC-RR0232 | Luc/RFP Reporter Cell Line-B16F10 | Inquiry |
CSC-RR0233 | mSlc5a5/Luc Reporter Cell Line-B16F10 | Inquiry |
CSC-RR0234 | Luc Reporter Cell Line-B16F10 | Inquiry |
CSC-RR0235 | mSlc5a5/GFP Reporter Cell Line-B16F10 | Inquiry |
CSC-RR0236 | mSlc5a5/RFP Reporter Cell Line-B16F10 | Inquiry |
CSC-RO0016 | mSlc5a5 Overexpression Stable Cell Line-B16F10 | Inquiry |
CSC-RR0528 | ZsYellow1 Reporter Cell Line - B16F10 | Inquiry |
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