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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | B16F10 is a murine melanoma cell line from the C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, then injecting them into fresh mice, a total of 10 times. B16F10 cells are highly metastatic and can form tumors and metastases post implantation into syngenic C57BL/6 mice or immunocompromised mice. |
| Strain | C57BL/6J |
| Tissue | Skin |
| Disease | Melanoma |
| Morphology | Mixture of spindle-shaped and epithelial-like cells |
| Gender | Unknown |
| Primary | No |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Applications | Cell culture / growth conditions, stable cell transfection, gene expression, protein expression, transient transfection |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Culture Conditions and Handling | |
| Thawing Frozen Cells |
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| Subculturing |
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| Subcultivation Ratio | The ratio of 1:10 is recommended. |
| Medium Renewal | Every 2 to 3 days |
| Freeze Medium | 93% culture medium + 7% DMSO> |
| Culture Temperature | 37°C |
| Incubation Condition | 5% CO2 |
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