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Creative Biogene innovates with Custom miRNA qPCR Primers Sets, offering profiling for miRNome expression, cancer, and disease-related miRNAs in humans and mice. These sets feature miRNA-specific primers arrayed into full miRNome panels, ensuring high content and relevance to biology or disease. Each primer undergoes experimental validation for a single dissociation curve peak and correct-sized amplification. MiqScript™ miRNA PCR arrays, available in 96-well and 384-well plates, are compatible with various real-time thermal cyclers. Positioned at the forefront of miRNA expression profiling, MiqScript™ sets are complemented by MiqScript™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits for optimal results.
Key Features of Our Custom miRNA qPCR Primers Set
| Cat# | Product Name | Species | Numbers of miRNA | Numbers of plates | Price |
| MRA001L | MiqScript™ human miRNome miRNA qPCR arrays | Human | 1,565 (miRBase V.17) | 19 x 96-well plates | Inquiry |
| MRA002L | MiqScript™ human miRNome miRNA qPCR arrays | Human | 1,700 (miRBase V.18) | 5 x 384-well plates | Inquiry |
| MRA003L | MiqScript™ human single-nucleotide mismatch miRNA qPCR arrays | Human | 61 | 1 x 96-well plates | Inquiry |
| MRA004L | MiqScript™ mouse miRNome miRNA qPCR arrays | Mouse | 834 (miRBase V.18) | 10 x 96-well plates | Inquiry |
| MRA005L | MiqScript™ mouse miRNome miRNA qPCR arrays | Mouse | 834 (miRBase V.18) | 3 x 384-well plates | Inquiry |
| Cat# | Product Name | Species | Numbers of miRNA | Numbers of plates | Price |
| MRA006L | MiqScript™ human cancer miRNA qPCR arrays | Human | 420 | 5 x 96-well plates | Inquiry |
| MRA007L | MiqScript™ human brain cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA008L | MiqScript™ human breast cancer miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA009L | MiqScript™ human ovarian cancer miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA010L | MiqScript™ human leukemia miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA011L | MiqScript™ human lung cancer miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA012L | MiqScript™ human bladder cancer miRNA qPCR arrays | Human | 79 | 1 x 96-well plates | Inquiry |
| MRA013L | MiqScript™ human colorectal cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA014L | MiqScript™ human endometrial cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA015L | MiqScript™ human gastric cancer miRNA qPCR arrays | Human | 80 | 1 x 96-well plates | Inquiry |
| MRA016L | MiqScript™ human hepatocellular carcinoma miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA017L | MiqScript™ human lymphoma miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA018L | MiqScript™ human melanoma miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA019L | MiqScript™ human head and neck cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA020L | MiqScript™ human pancreatic cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA021L | MiqScript™ human prostate cancer miRNA qPCR arrays | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA022L | MiqScript™ mouse breast cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA023L | MiqScript™ mouse brain cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA024L | MiqScript™ mouse ovarian cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA025L | MiqScript™ mouse prostate cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA026L | MiqScript™ mouse colorectal cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA027L | MiqScript™ mouse hepatocellular carcinoma miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA028L | MiqScript™ mouse lung cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA029L | MiqScript™ mouse melanoma qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA030L | MiqScript™ mouse pancreatic cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA031L | MiqScript™ mouse head and neak cancer miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| MRA032L | MiqScript™ mouse leukemia miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
| Cat# | Product Name | Species | Numbers of miRNA | Numbers of plates | Price |
| MRA033L | MiqScript™ human inflammatory miRNA qPCR array | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA034L | MiqScript™ human heart disease miRNA qPCR array | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA035L | MiqScript™ human immunopathology miRNA qPCR array | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA036L | MiqScript™ human IPS (Stem cell) miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA037L | MiqScript™ human muscle disease miRNA qPCR array | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA038L | MiqScript™ human toxicology related miRNA qPCR array | Human | 84 | 1 x 96-well plates | Inquiry |
| MRA039L | MiqScript™ human serum and plasma miRNA qPCR arrays | Human | 168 | 2 x 96-well plates | Inquiry |
| MRA040L | MiqScript™ mouse inflammatory miRNA qPCR arrays | Mouse | 84 | 1 x 96-well plates | Inquiry |
Custom miRNA qPCR Primers Sets enable precise quantification of miRNA expression through qPCR technology. Using custom-designed primers arrayed into comprehensive panels, these sets cover miRNome expression, cancer, and disease-related miRNAs. The experiment ensures accuracy with a single dissociation curve peak and correct-sized amplification for targeted miRNAs. Custom miRNA qPCR Primers Sets from Creative Biogene have versatile applications in miRNA expression profiling：
Case Study 1
TGF-β1 is a versatile cytokine with the ability to either stimulate or impede the development and progression of cancer. The researcher employed human miRNome miRNA qPCR arrays to investigate the impact of TGF-β1 on the global miRNA profile in renal cell carcinoma (RCC). The study revealed that TGF-β1 orchestrates a complex network involving miRNAs and mRNAs associated with RCC transformation. Notably, TGF-β1 modulates the proliferation of RCC cells and alters the expression of oncogenic regulators, including ETS1. Furthermore, TGF-β1 influences the expression of specific miRNAs, such as miR-30c-5p, miR-155-5p, miR-181a-5p, and miR-181b-5p. This reciprocal regulation suggests a novel feedback mechanism mediated through miRNAs, highlighting the multifaceted roles of ETS1 in different subtypes of RCC tumors.
Figure 1. The researcher utilized human miRNome miRNA qPCR arrays to analyze global miRNA changes post-TGF-β1 treatment in 786-O cells, identifying alterations in 15 microRNAs. (Hanusek K, et al., 2022)
Case Study 2
The researcher employed human miRNome miRNA qPCR arrays to investigate the impact of Human Cytomegalovirus (HCMV) and Human Herpesvirus 6 (HHV-6) infections on the microRNome (miRNome) of primary human dermal fibroblasts. This in vitro study, utilizing Taqman arrays detecting 754 microRNAs, revealed significant modulation of miRNA expression by both herpesviruses. The observed deep modulation of key fibrosis-associated miRNAs suggests a potential role of HCMV and HHV-6 in the pathogenesis of fibrosis in systemic sclerosis. Identifying specific miRNAs may offer biomarkers for diagnosis, disease progression assessment, and potential antifibrotic therapies in systemic sclerosis.
Figure 2. The researcher employed TaqMan Advanced miRNA Human A and B Cards for microarray analysis, examining the expression of 754 human microRNAs in HCMV-infected versus uninfected cells. Heatmaps illustrate the up-regulated (blue) and down-regulated (red) values, providing a comprehensive view of microRNA expression changes. (Soffritti I, et al., 2021)
Q: What are the primary features of the Custom miRNA qPCR Primers Set?
A: The primary features of the Custom miRNA qPCR Primers Set include high sensitivity and accuracy, broad linearity and high reproducibility, comprehensive coverage of miRNome, cancer and disease-related miRNAs, compatibility with multiple real-time thermal cyclers, and convenience offered by ready-to-use MiqScript™ miRNA PCR Arrays.
Q: In what applications can the Custom miRNA qPCR Primers Set be utilized?
A: The Custom miRNA qPCR Primers Set can be used for comprehensive miRNA expression analysis, disease research particularly in cancer and cardiovascular diseases, toxicology studies assessing the impact of environmental toxins on miRNA expression, and stem cell investigations specifically into the regulation of miRNA in induced pluripotent stem cells (iPSCs).
Q: How does the Custom miRNA qPCR Primers Set ensure the accuracy of miRNA quantification?
A: The Custom miRNA qPCR Primers Set ensures the accuracy of miRNA quantification through a single dissociation curve peak and correct-sized amplification for each targeted miRNA.
Q: What makes the MiqScript™ Technology in the Custom miRNA qPCR Primers Set special?
A: The MiqScript™ Technology, integrated into the Custom miRNA qPCR Primers Set, provides ready-to-use miRNA PCR arrays in 96-well and 384-well plates, making it efficient and convenient for users. This technology allows comprehensive miRNA profiling and is complemented by MiqScript™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits for optimal results.
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