PC-9 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	PC-9, formerly known as PC-14, is a human non-small cell lung cancer (NSCLC) cell line that harbors a deletion mutation in exon 19 of the EGFR gene. It is derived from an undifferentiated human adenocarcinoma of the lung tissue and consists of a heterogeneous mixture of round and spindle cells. PC-9 cells exhibit both adherent and suspended growth characteristics. PC-9 cells have a variety of applications in cancer research. They are used to study the effects of alpha-tocopherol on cytotoxic drugs to investigate whether it enhances or diminishes their efficacy against NSCLC. PC-9 cells serve as a model to study resistance of lung cancer to tyrosine kinase inhibitors (TKIs), which helps understand the mechanisms involved. In addition, they are used in tumorigenicity studies to assess the tumorigenic potential and behavior of NSCLC.
Tissue	Lung
Disease	Adenocarcinoma
Morphology	Heterogeneous mixture of round cells and spindle shaped cells.
Gender	Male
Age	45 years
Product Format	Frozen
Growth Mode	Mixed, adherent and suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Drug Development and Screening 3. EGFR Mutation Studies 4. Molecular and Cellular Pathways 5. Resistance Mechanisms 6. Apoptosis and Cell Cycle Research
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Not specified
Tumorigenic	Yes
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes used in this protocol are for 75 cm2 flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Transfer the medium containing floating cells to a centrifuge tube. 2. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently aspirate the cells. 5. To remove the trypsin-EDTA solution, transfer the cell suspension to the centrifuge tube containing the medium and cells from step 1 and spin at approximately 125 x g for 5 to 10 minutes. 6. Discard the supernatant and resuspend the cells in fresh growth medium. 7. Add the appropriate cell suspension to a new culture vessel. Seed the cells at a density of 2-4 x 10,000 cells/cm2. 8. Place the culture vessel in a 37℃ incubator.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	The ratio of 1:8 is recommended.
Culture Medium	RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum.
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	95% FBS + 5% DMSO (Dimethyl sulfoxide)

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Cat.No.	Product Name	Price
CSC-RO0456	Human EGFR-Del19/T790M/C797S Stable Cell Line - PC-9	Inquiry
CSC-RO0716	Human EGFR-Del19-C797S Stable Cell Line - PC-9	Inquiry
CSC-RR01052	mScarlet/LUC Reporter Cell Line - PC-9	Inquiry
CSC-RR0512	GFP Reporter Cell Line - PC-9	Inquiry
CSC-RR0513	Luciferase Reporter Cell Line - PC-9	Inquiry
CSC-RR0514	GFP/Luc Reporter Cell Line - PC-9	Inquiry

* For research use only. Not intended for any clinical use.

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