BAC Library Screening Service

Creative Biogene is one of the leading biotechnology companies specializing in BAC library screening service. Creative Biogene's sophisticated equipment, advanced technologies and highly experienced staffs are available to assist you with the comprehensive PCR-based BAC library screening to help you select positive BAC clones which contain target segment. Creative Biogene's goal is to provide you with the most affordable, high-quality BAC library screening service to ensure your satisfaction in a timely and professional manner.

Fig.1 Retrieving procedure of BAC clones of interest from the non-gridded library pools. [3]

Bacterial artificial chromosome (BAC) library has been widely used due to its advantages such as large inserting size, strong stability, and easy to operate. Besides, BAC libraries have comparative advantages over yeast artificial chromosome (YAC) libraries as regards a low rate of chimera formation and easily manipulated individual clones. BAC libraries serve as an essential resource for positional gene cloning, sequence analysis of genomes, physical mapping, and comparative genomics. An operation common to virtually all applications of BAC libraries is screening the libraries for clones harboring specific nucleotide sequences. Two major approaches, PCR-based and hybridization-based strategies, have been used in BAC library screening. However, hybridization-based screening approaches have several limitations over PCR-based screening methods. The presence of repeat elements in the labeled probe often confounds hybridization results, and the procedures are more cumbersome since they involve radioactive material. Thus, PCR-based screening of large inserts libraries now as an alternative choice to hybridization-based screens.

Creative Biogene, as one of the leading biotechnology service vendor in the world, is able to provide you the comprehensive PCR-based BAC library screening service with advanced platform, quick turnaround, and uncompromising quality results. Creative Biogene is a reliable partner that can work with you to accelerate the speed to achieve your research goals. If you have any special requirements in our BAC library screening service, please feel free to contact us. We are looking forward to working together with your attractive projects.

References:

Yim YS, et al. (2007). A BAC pooling strategy combined with PCR-based screenings in a large, highly repetitive genome enables integration of the maize genetic and physical maps. BMC genomics, 8(1), 47.
Feng D, et al. (2011). PCR-based screening of BAC clones of different chromosomes in Chinese cabbage. Frontiers of Agriculture in China, 5(4), 524-528.
Xia Z, et al. (2014). Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library. Analytical biochemistry, 444, 38-40.

* For research use only. Not intended for any clinical use.

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