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Somatic cells transform into induced pluripotent stem (iPS) cells using adenoviral vectors, avoiding ethical concerns of embryonic stem (ES) cell research. Unlike traditional methods using retrovirus or lentivirus, adenoviral vectors prevent integration into the host cell genome, mitigating mutation and transcription interference risks. Successful studies demonstrate adenoviral-produced iPS cells without viral or transgene integration into chromosomal DNA. Creative Biogene provides adenoviral vectors expressing various transcription factors that are commonly used in the development of iPS cells such as c-Myc, Klf4, Oct4, and Sox2.
Key Features of Our iPS Adenovirus Particles
iPS Adenovirus Particles Product List
Adenoviral reprogramming factors enable efficient pluripotency induction without genomic modification, ensuring safety through transient extrachromosomal vectors. The adenoviral approach, in contrast to integrative methods, produces safer integration-free iPSCs, making it ideal for downstream research and therapeutic applications. Explore the possibilities with our innovative products:
Case Study 1
KLF14's role in liver fibrosis was explored using human, rat, and mouse fibrotic models. KLF14 overexpression inhibited HSC activation, inducing apoptosis and G2/M arrest. Mechanistically, KLF14 transactivated PPARγ promoter activity. EZH2-mediated histone modification regulated KLF14 expression. Adenovirus-mediated KLF14 overexpression alleviated TAA-induced rat liver fibrosis in a PPARγ-dependent manner. The EZH2 inhibitor EPZ-6438 showed therapeutic effects. KLF14 downregulation in human liver fibrosis correlated with EZH2 upregulation and PPARγ downregulation, suggesting the EZH2/KLF14/PPARγ axis as a potential therapeutic target.
Figure 1. In the investigation of KLF14's role in hepatic fibrogenesis in vivo, KLF14 expression was enforced in a TAA-induced rat liver fibrosis model using adenovirus injection via the tail vein. (Du Z, et al., 2021)
Case Study 2
Researchers utilized adenovirus-mediated gene manipulation to investigate the role of Myc in pancreatic β-cell expansion during metabolic stress. Specifically, they induced insulin resistance in young mice through a short-term high-fat diet (HFD). β-cell-specific Myc knockout mice failed to adaptively expand, exhibiting impaired glucose tolerance and dysfunction. Key regulators, including PKCζ, ERK1/2, mTOR, and PP2A, were identified in the Myc response. DNA methylation analysis revealed hypomethylation of cell cycle genes targeted by Myc in islets from young mice on a short-term HFD. However, this epigenetic response was absent in aged mice, suggesting a resistance to Myc action with aging. The study highlights Myc's critical role in β-cell expansion during metabolic stress in young mice and unveils potential age-related epigenetic modifications affecting Myc function.
Figure 2. The researchers employed Myc-adenovirus to investigate its role in pancreatic β-cell replication, expansion during metabolic stress-induced insulin resistance, and the regulatory mechanisms involving PKCζ, ERK1/2, mTOR, and PP2A. (Rosselot C, et al., 2019)
Q: What is the primary function of iPS Adenovirus Particles in research?
A: iPS Adenovirus Particles are designed to deliver genes associated with induced pluripotent stem cells (iPSCs), allowing researchers to induce reprogramming in somatic cells.
Q: Which specific genes are typically delivered by iPS Adenovirus Particles?
A: iPS Adenovirus Particles commonly carry key pluripotency-associated genes such as Oct4, Sox2, Klf4, and c-Myc, essential for cellular reprogramming.
Q: What are the advantages of using iPS Adenovirus Particles for iPSC generation?
A: iPS Adenovirus Particles offer a robust and efficient method for inducing cellular reprogramming in somatic cells. Unlike some methods, adenovirus-based reprogramming avoids genomic integration of transgenes.
Q: Can iPS Adenovirus Particles be used for gene delivery in other cell types beyond iPSC generation?
A: While designed for iPSC generation, iPS Adenovirus Particles can be adapted for delivering specific genes in various cell types for functional studies beyond reprogramming.
| Cat.No. | Product Name | Price |
|---|---|---|
| AD00036Z | Human OCT4 adenoviral particles | Inquiry |
| AD00037Z | Human Sox2 adenoviral particles | Inquiry |
| AD00038Z | Human myc adenoviral particles | Inquiry |
| AD00040Z | Human OCT4,Sox2,KLF4 adenoviral particles | Inquiry |
| AD00194Z | Human KLF14 adenoviral particles | Inquiry |
| AD00195Z | Human KLF4 adenoviral particles | Inquiry |
| AD00196Z | Human KLF5 adenoviral particles | Inquiry |
| AD00362Z | NANOG adenovirus | Inquiry |
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