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Fiber-Modified Ad5-RGD Adenovirus Production

Adenoviruses based on serotype 5 (Ad5) have been widely used to deliver transgenes to different cells and tissues. Ad5 cell infection is based on the recognition of Coxsackievirus–adenovirus receptor (CAR) expressed on cell surface, and the fiber is the key mediator for the recognition. Due to the nature of Ad5 infection, cells with no or low CAR expression levels, can not be efficiently infected by Ad5. To overcome this problem, scientists have developed CAR-independent adenoviruses through modifying the Ad5 fiber. RGD (Arg-Gly-Asp) peptide can bind to integrins and has been incorporated into Ad5 fiber to enhance virus infection via CAR-independent pathway. RGD-fiber modified adenoviruses (Ad5-RGD) have been successfully used to transduce several cells and tissues, including macrophages, T cells.

Fiber-Modified Ad5-RGD Adenovirus ProductionFigure 1. RGD-fiber modified adenovirus (Ad5-RGD)

Creative Biogene has over 10 years of experience in the field of adenovirus. Our scientists have established a series of standard operating procedures (SOPs) to produce adenoviruses with high titer, purity, viability and consistency. In addition to classic adenovirus production, we can also produce Ad5-RGD virus by introducing Arg-Gly-Asp (RGD) peptide into the HI loop of the Ad5 fiber knob domain.

Applications

-Infection of cells or tissues with integrin expressing

-In vitro or in vivo gene delivery

Features

-One-stop solution: from DNA synthesis to Ad5-RGD production

-Produce adenovirus particles in high titer

-Rigorous quality controls

To get more information about what we can offer, please contact us at 1-631-626-9181 or .

Reference:

  1. Wu H, Seki T, Dmitriev I, Uil T, Kashentseva E, Han T, Curiel DT. Double modification of adenovirus fiber with RGD and polylysine motifs improves coxsackievirus-adenovirus receptor-independent gene transfer efficiency. Hum Gene Ther. 2002 Sep 1;13(13):1647-53.
For research use only. Not intended for any clinical use.

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