Lipopolysaccharides (LPS), also known as endotoxins, are large molecules consisting of a lipid and a polysaccharide...
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Lipopolysaccharides (LPS), also known as endotoxins, are large molecules consisting of a lipid and a polysaccharide composed of O-antigen outer core and inner core joined by a covalent bond (Figure 1). They are found in the outer membrane of Gram-negative bacteria and elicit strong immune responses in normal mammalian cells. LPS is heat stable endotoxin and has long been recognized as a crucial factor in septic shock (septicemia) in humans. The lipid A moiety has been identified as key to the endotoxin activity of LPS.
Figure 1. Bacterial endotoxin (LPS).
LPS preparations have been used in research for the elucidation of LPS structure, metabolism, physiology, immunology, toxicity, and biosynthesis. Moreover, they have also been used to induce synthesis and secretion of growth promoting factors such as interleukins. Due to its connection to septicemia, lipopolysaccharide has been studied to identify possible targets for antibodies and inhibitors to LPS biosynthesis.
LPS Extraction Kit is a product which can separate and purify LPS present in the outer wall of Gram-negative bacteria. Natural endotoxin consists of LPS, phospholipids and proteins. It is very stable in its natural state, negatively charged, strong in heat, and has a large molecular weight. The common methods of hot phenol or oil/phenol/chloroform for purifying these molecules are both complex and time-consuming. Creative Biogene’s LPS Extraction Kit offers a new method for extracting LPS with higher yields, fewer steps and in far less time. Based on a robust water/chloroform/phenol extraction technology, the LPS extraction kit is flexible enough to run small culture volumes (<5 ml) or large (>100 ml) with similar yields.