AI-Driven Enzyme Engineering

Creative Biogene is a global leader in genetic research products and services, trusted by researchers from over 40 countries. We have assisted in publishing over 300 patents and scientific articles in the last five years. Creative Biogene is a leader in the field of AI-driven enzyme engineering services. By utilizing AI technology, we optimize enzyme function and offer customized, effective solutions that enable significant progress in various research areas.

Natural Properties of Enzymes and Design Challenges

Enzymes play a crucial role in biomanufacturing processes and are significant products of biomanufacturing themselves. In 2023, the global market value for industrial enzymes alone reached $7.4 billion. As catalytic biomolecules, enzymes have developed specific functions through natural selection, such as activity, stability, and substrate selectivity. However, the sequence space of enzymes is calculated on the order of 20^N (where N is the length of the primary protein sequence). Even after millions of years of evolution, only a small fraction of this sequence space has been explored by nature. Consequently, one of the core tasks of enzyme engineering is to introduce mutations or new structural domains to enhance specific properties, meeting industrial or laboratory needs.

Limitations of Traditional Methods

In the mid-20th century, Lerner introduced mutations in bacteria using chemical mutagenesis. However, this method lacked targeted control, resulting in high randomness and low efficiency. With the advancement of recombinant protein technology, amino acids in the primary protein sequence could be precisely controlled and edited. Using prior knowledge of the working mechanisms and physicochemical properties at the protein level, researchers could engage in rational design, assessing whether specific amino acid substitutions would enhance protein properties or modify functional specificity. Nevertheless, this method relies heavily on detailed knowledge of protein structures and mechanisms, rendering it ineffective for proteins with unknown structures or mechanisms.

Directed evolution strategies have accelerated the evolution of proteins towards specific targets through random mutagenesis and high-throughput screening. This approach bypasses the knowledge barriers of rational design, as it does not require understanding protein structures or mechanisms. However, it still involves high randomness and requires extensive screening, resulting in relatively low efficiency.

Significance of AI in Enzyme Design

As a data-driven computational approach, artificial intelligence (AI) significantly enhances the efficiency and success rate of enzyme design. By guiding appropriate sampling methods, deep neural network models can provide highly reliable recommendations for modifying enzyme functions, such as activity, thermal stability, and selectivity. Currently, AI-assisted enzyme engineering is in a rapid development phase. Various neural network models are continuously updated and iterated, evolving from early manual features to learning native information based on protein sequences, multiple sequence alignments (MSA), and structures. This progress highlights AI's advantages in handling high-dimensional information, especially given the exponential growth of protein sequence databases due to large-scale gene sequencing. In the future, AI methods in enzyme engineering must achieve greater breakthroughs in prediction accuracy and understanding protein properties. The development of high-performance neural network models will be a key tools for biochemical laboratories to reduce the costs and time associated with directed evolution.

Service Overview

Creative Biogene's AI-assisted precision enzyme engineering service is grounded in high-quality datasets. We leverage deep learning and molecular dynamics models, combined with our extensive enzyme database platform and high-performance computing capabilities, to drive the standardization and precision of enzyme design. Creative Biogene offers comprehensive AI-driven enzyme engineering services, including precise enzyme structure prediction and pocket analysis, efficient construction of optimized enzyme libraries, directed evolution and optimization using advanced AI technologies, customized enzyme design for industrial applications, and high-throughput screening for rapid validation of enzyme variants.

Prediction

Discovery

Optimization

Design

Validation

Enzyme Structure Prediction and Pocket Analysis

Enzyme structure prediction and pocket analysis services leverage AI-driven models for precise predictions, including enzyme structure, ligand binding simulations, and chemical modifications.

Enzyme Library Construction

Creative Biogene specializes in AI-driven enzyme library construction, offering efficient and precise solutions for designing and optimizing diverse enzyme libraries using advanced artificial intelligence technologies, enhancing process efficiency, and accelerating research and development in industrial biotechnology.

Directed Evolution and Enzyme Optimization

Creative Biogene's Directed Evolution and Enzyme Optimization service utilizes advanced AI and deep learning technologies to enhance enzyme performance through comprehensive mutation analysis and iterative optimization, ensuring tailored solutions for diverse industrial and pharmaceutical applications.

AI-Driven Enzyme Design

Creative Biogene's AI-driven enzyme design service uses advanced AI technology to revolutionize protein engineering. We integrate computational modeling, deep learning algorithms, and advanced molecular simulations to design customized functional enzymes that meet diverse industrial needs.

High-throughput screening and Experimental Validation

Creative Biogene's AI-assisted high-throughput screening service integrates advanced computational algorithms with AI technology to efficiently identify high-activity enzyme mutations, enhancing enzyme engineering success rates and overcoming traditional screening limitations.

Creative Biogene leads in global AI-driven enzyme engineering services, optimizing enzyme functions with advanced artificial intelligence technology to deliver customized solutions, advancing research and industrial innovation. Accelerate your research journey with Creative Biogene's trusted expertise and innovation. Contact us to embark on the forefront of scientific exploration!

* For research use only. Not intended for any clinical use.

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