Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The Capan-1 cell line was derived from human pancreatic adenocarcinoma and established from the ascitic fluid of a 40-year-old Caucasian male. It was first characterized in 1975 and is known for its ductal epithelial morphology, which closely resembles primary pancreatic tumors. This cell line has been praised for its ability to produce mucins, which are characteristic of many pancreatic adenocarcinomas, and thus may serve as a model for mucinous pancreatic cancer. These cells have a single base pair deletion in the BRCA2 allele, resulting in the expression of a truncated and dysfunctional protein. Additionally, they possess oncogenic mutations in K-Ras (G12V) and inactivating p53 mutations. These cells express elevated levels of epidermal growth factor receptor (EGFR) but do not express SMAD4 protein (i.e., SMAD4 deficiency). These mutations make the Capan-1 cell line a valuable tool for studying the molecular mechanisms of pancreatic cancer and for the preclinical evaluation of new therapeutics targeting these pathways. Additionally, Capan-1 cells can be used to study the biology of pancreatic cancer stem cells, providing insights into the behaviors that lead to cancer recurrence and resistance to conventional therapies. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 40 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and development 3. Genetic studies 4. Signal transduction pathways 5. Biomarker identification 6. Immunotherapy research 7. Metabolism studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Capan-1 tumors had the highest levels of alpha-smooth muscle actin (an indicator of cancer-associated fibroblasts) and collagen. This suggests that Capan-1 is a suitable model to study MUC4-targeted therapy. The Capan-1 cell line was used to create the CDX (cell line derived xenograft) Capan-1 xenograft mouse model. The Capan-1 xenograft model has been used to study pancreatic cancer treatments (lapatinib, S-1, small molecule inhibitors, immunotherapy, etc.). |
| Characteristics | |
| Protein Expression | p53 negative |
| Antigen Expression | Blood Type A, Rh+ |
| Karyotype | (P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes, and minute marker |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Culture medium 95%; DMSO, 5% |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00619 | GFP/Luc Reporter Cell Line - Capan-1 | Inquiry |
| CSC-RR0432 | GFP Stable Cell Line - Capan-1 | Inquiry |
| CSC-RR0614 | Luciferase Reporter Cell Line - Capan-1 | Inquiry |
Copyright © Creative Biogene. All rights reserved.