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In genomics, synthetic biology, and precision medicine, large-scale oligonucleotide synthesis is a foundational technology. Contemporary studies increasingly require libraries of thousands to millions of precisely designed oligos, demanding platforms that ensure high fidelity, uniformity, and scalability.
Advances in microarray-based synthesis and electrochemical control have reduced costs, yet maintaining sequence accuracy and reproducibility across large oligo pools remains challenging. Creative Biogene addresses these needs with a customizable Oligo-Pool Synthesis Service, providing high-quality, high-complexity libraries suitable for diverse genomic and synthetic biology applications.
Building upon years of expertise in DNA synthesis and synthetic biology, Creative Biogene has developed a robust and efficient oligo-pool synthesis workflow designed for precision, scalability, and flexibility. Our technology integrates advanced microarray synthesis, electrochemical control, and chip-based parallel synthesis, allowing thousands of unique sequences to be produced simultaneously on a single chip with exceptional accuracy.
Creative Biogene offers two chip configurations to accommodate projects of different scales:
This high-throughput capacity dramatically reduces cost per sequence and shortens project timelines—making large library projects more accessible and affordable than ever before.
Quality is paramount in oligo synthesis. Our process employs tightly controlled electrochemical coupling reactions, optimized reagent formulations, and multi-step error correction strategies to ensure maximum sequence integrity.
Through rigorous process control and enzymatic error repair, we achieve an average error rate below one base per 200 nucleotides, while minimizing sequence dropouts and bias. This results in highly uniform and reliable pools that maintain consistency across replicates and downstream applications.
We support a wide range of oligo lengths—from 10 nucleotides up to 200 nucleotides or more—to accommodate diverse research goals. Whether you are designing short probes, variable linker sequences, or longer fragments for gene assembly, our synthesis system provides the flexibility you need.
Custom adapters, barcodes, or cloning handles can be incorporated into your oligos to streamline downstream workflows such as PCR amplification, cloning, or next-generation sequencing (NGS).
Compared to conventional single-oligo synthesis, Creative Biogene's pool-based synthesis offers an unparalleled cost advantage—particularly for large-scale projects requiring thousands of sequences.
Optimized manufacturing pipelines allow rapid turnaround times of 2–4 weeks, depending on library complexity and purification requirements, ensuring you get results faster without compromising quality.
From concept to delivery, our experienced team provides comprehensive support throughout the project lifecycle:
Whether you supply ready-to-synthesize sequences or need assistance with design, our experts ensure your oligo pool performs exactly as intended.
Creative Biogene provides multiple delivery options to suit your downstream applications, including:
All oligos are delivered with detailed QC documentation to ensure traceability and confidence in every sequence.
Our oligo-pool synthesis follows a rigorously optimized and quality-controlled workflow to ensure accuracy, purity, and consistency:
1Project Assessment & Design: Clients provide sequences, pool size, oligo length, and application. Our team optimizes sequence design, thermodynamic parameters, and incorporates adapters or barcodes as needed.
2Chip Layout & Preparation: Sequences are mapped to the microelectrode array, ensuring even distribution and minimal cross-reactivity.
3Electrochemical Synthesis: Each microelectrode independently controls nucleotide coupling under precise voltage and reagent regulation to synthesize the oligos.
4Deprotection & Purification: Oligos are released from the array, protective groups removed, and pools purified to remove truncated fragments or contaminants, with optional PAGE/HPLC purification.
5Quality Control & Delivery: NGS validation ensures sequence accuracy and uniformity. Purified oligos are delivered in the requested format with full QC documentation and post-delivery technical support.
Whether your project involves CRISPR screening, variant library construction, FISH probe synthesis, DNA assembly, or next-generation spatial genomics, Creative Biogene offers the integrated oligo-pool solution you need to move from design to discovery—efficiently, reliably, and affordably. Partner with Creative Biogene today to unlock the full potential of oligo-pool technology.
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