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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The MV-4-11 cell line, isolated from blasts from children with biphenotypic B myelomonocytic leukemia, is an important resource for the study of acute leukemias, particularly acute myeloid leukemia (AML). MV4-11 cells are characterized by a high proliferation rate and the presence of certain genetic abnormalities. A translocation between chromosomes 4 and 11 results in the generation of the MLL-AF4 fusion gene, which plays a critical role in leukemogenesis and contributes to the aggressive nature of leukemias. The presence of the MLL-AF4 fusion gene makes these cells particularly important for the study of understanding the molecular mechanisms of leukemogenesis and of targeted therapies aimed at disrupting the function of this oncogenic fusion protein. This cell line has further utility in metabolomic and transcriptomic studies, providing a comprehensive understanding of metabolic alterations and redox adaptations in leukemia. |
| Tissue | Peripheral blood |
| Disease | Biphenotypic B Myelomonocytic Leukemia |
| Morphology | Lymphoblast |
| Gender | Male |
| Age | 10 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Drug testing 2. Genetic studies 3. Molecular and cellular biology 4. Studying FLT3 mutations 5. Antibody production 6. Protein expression studies 7. Disease modelling |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The MV4-11 cell line was established by Rovera and colleagues from blasts from a 10-year-old male with biphenotypic B myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), is required for establishment of this cell line and is required for its continued proliferation in chemically defined media. |
| Characteristics | |
| Karyotype | 48, xY, t(4,11)(q21,q23), +8, +19 |
| Antigen Expression | CD4 (40-96%); CD10 (4-11%); CD15 (96-99%) |
| Mutational Profile | FLT3 mut |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Cultures can be maintained by adding or replacing fresh medium. Start culture at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. It is recommended to use T-75 flasks for passage of this product. |
| Medium Renewal | Every 2 to 3 days |
| Culture Medium | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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