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HeLa Cell Line


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HeLa Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionA derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in a number of subsequently derived lines, due to poor culture techniques. Susceptible to Poliovirus type I and adenovirus type 3.
Tissue of OriginHuman cervix carcinoma
Age31 years adult
GenderFemale
EthnicityBlack
Cell TypeEpithelial
Product FormatFrozen
Growth ModeAdherent
Biosafety Level2 [Cells contain Adenovirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsThese cells are a suitable transfection host. And the cell line can be used to screen for Escherichia coli strains with invasive potential.
Shipped inDry ice
Storage Temperature−196°C
ReferencesCancer Res 1952;12:264; Proc Soc Exp Biol Med 1954;87:480
Characteristics
KaryotypeModal number = 82; range = 70 to 164; aneuploid
The literatures have reported four typical HeLa marker chromosomes. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 consists of the short arm of chromosome 3 and the long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 is a combination of the long arm of chromosome 11 and an arm of chromosome 19.
ImagesHeLa Cell Line
CommentsHeLa cells are positive for keratin by immunoperoxidase staining.
The cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
It has reported that P53 expression is low, and normal levels of pRB (retinoblastoma suppressor) were found.
Genes ExpressedLysophosphatidylcholine (lyso-PC) induces c-jun N-terminal kinase activity (JNK1) and AP-1 activity by a protein kinase C-independent pathway. The cells are positive for keratin by immunoperoxidase staining.
Virus SusceptibilityHuman adenovirus 3; Encephalomyocarditis virus; Human poliovirus 1; Human poliovirus 2; Human poliovirus 3
Culture Conditions and Handling
Culture MediumTo make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes are given for a 75 cm2 flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation RatioThe ratio of 1:2 to 1:6 is recommended.
Fluid Renewal2 to 3 times per week
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereAir, 95%; carbon dioxide (CO2), 5%
Cat.No. Product Name Price
CSC-RR0020Human NF-kB Prom/Luciferase/Hyg Reporter Stable Cell Line-HelaInquriy
CSC-RR0028Human NR3C1 Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLaInquriy
CSC-RR0032Human NFAT Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLaInquriy
CSC-RR0034Human STAT1 Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLaInquriy
CSC-RR0035Human SRF Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLaInquriy
CSC-RR0036Human TAD Kinase Prom/Luciferase/Hyg Reporter Stable Cell Line-HeLaInquriy
CSC-RG1745Human F2RL1 Stable Cell Line-HeLaInquriy
CSC-RN0007Mouse MMTV Stable Cell Line-HeLa (bla)Inquriy
CSC-RR0302HRE-Luc Reporter Cell Line-HeLaInquriy
CSC-RR0305TLR4/Luc Reporter Cell Line-HeLaInquriy
CSC-RR0306p53-Luc Reporter Cell Line-HeLaInquriy
CSC-RR0315ATF6-Luc Reporter Cell Line-HeLaInquriy
CSC-RR0377Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0394STAT3 Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0398GR Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0399AP-1 Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0402TCF/LEF Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0407ELK-TAD Luciferase Reporter Cell Line - HeLaInquriy
CSC-RR0047GFP Reporter Stable Cell Line-HeLaInquriy
CSC-RR0089tGFP Reporter Stable Cell Line-HeLaInquriy
CSC-RK0002Human ERCC1 Knockdown Cell Line-HeLaInquriy
CSC-RK0003Human XPF Knockdown Cell Line-HeLaInquriy
CSC-RK0005Human hHR23A Knockdown Cell Line-HeLaInquriy
CSC-RK0006Human hHR23B Knockdown Cell Line-HeLaInquriy
CSC-RK0007Human KIN17 Knockdown Cell Line-HeLaInquriy
CSC-RK0008Human LigIII Knockdown Cell Line-HeLaInquriy
CSC-RK0009Human LigIV Knockdown Cell Line-HeLaInquriy
CSC-RK0010Human MRE11 Knockdown Cell Line-HeLaInquriy
CSC-RK0011Human NBS1 Knockdown Cell Line-HeLaInquriy
CSC-RK0012Human Rad50 Knockdown Cell Line-HeLaInquriy
CSC-RK0014Human XPA Knockdown Cell Line-HeLaInquriy
CSC-RK0015Human XPC Knockdown Cell Line-HeLaInquriy
CSC-RK0016Human XRCC1 Knockdown Cell Line-HeLaInquriy
CSC-RK0017Human XRCC4 Knockdown Cell Line-HeLaInquriy
CSC-RK0018Human PARP1 Knockdown Cell Line-HeLaInquriy
CSC-RK0019Human PARP2 Knockdown Cell Line-HeLaInquriy
CSC-RK0020Human XLF Knockdown Cell Line-HeLaInquriy
CSC-RK0021Human hRad54 Knockdown Cell Line-HeLaInquriy
CSC-RK0022Human MSH2 Knockdown Cell Line-HeLaInquriy
CSC-RK0023Human p53 Knockdown Cell Line-HeLaInquriy
CSC-RK0024Human ATR Knockdown Cell Line-HeLaInquriy
CSC-RK0025Human BLM Knockdown Cell Line-HeLaInquriy
CSC-RK0026Human FancD2 Knockdown Cell Line-HeLaInquriy
CSC-RK0027Human BRCA2 Knockdown Cell Line-HeLaInquriy
CSC-RK0028Human ATM Knockdown Cell Line-HeLaInquriy
CSC-RK0029Human MTERFD2 Knockdown Cell Line-HeLaInquriy
CSC-RK0030Human BRCA1 Knockdown Cell Line-HeLaInquriy
CSC-RK0031Human ATP6AP2 Knockdown Cell Line-HeLaInquriy
CSC-RK0032Human HIF1A Knockdown Cell Line-HeLaInquriy
CSC-RK0033Human ABL1 Knockdown Cell Line-HeLaInquriy
CSC-RK0034Human NCL Knockdown Cell Line-HeLaInquriy
CSC-RK0035Human HMGB1 Knockdown Cell Line-HeLaInquriy
CSC-RK0036Human PCNA Knockdown Cell Line-HeLaInquriy
CSC-RK0037Human NEIL1 Knockdown Cell Line-HeLaInquriy
CSC-RK0038Human TRIO Knockdown Cell Line-HeLaInquriy
CSC-RK0039Human BCL2 Knockdown Cell Line-HeLaInquriy
CSC-RK0040Human TNFR1 Knockdown Cell Line-HeLaInquriy
CSC-RK0254Human ADAM17 Knockdown Cell Line-HeLaInquriy
CSC-RK0255Human AKT1 Knockdown Cell Line-HeLaInquriy
CSC-RK0256Human EIF2C2 Knockdown Cell Line-HeLaInquriy
CSC-RK0257Human BAX Knockdown Cell Line-HeLaInquriy
CSC-RK0258Human BID Knockdown Cell Line-HeLaInquriy
CSC-RK0259Human BTG2 Knockdown Cell Line-HeLaInquriy
CSC-RK0260Human CASP3 Knockdown Cell Line-HeLaInquriy
CSC-RK0261Human CASP9 Knockdown Cell Line-HeLaInquriy
CSC-RK0262Human CDK2 Knockdown Cell Line-HeLaInquriy
CSC-RK0263Knockdown Control Cell Line-HeLaInquriy
CSC-RK0264Human PTGS2 Knockdown Cell Line-HeLaInquriy
CSC-RK0265Human CTNNB1 Knockdown Cell Line-HeLaInquriy
CSC-RK0266Human DIABLO Knockdown Cell Line-HeLaInquriy
CSC-RK0267Human PRKDC Knockdown Cell Line-HeLaInquriy
CSC-RK0268Human XPO5 Knockdown Cell Line-HeLaInquriy
CSC-RK0269Human FADD Knockdown Cell Line-HeLaInquriy
CSC-RK0270Human FEN1 Knockdown Cell Line-HeLaInquriy
CSC-RK0271Human GNAQ Knockdown Cell Line-HeLaInquriy
CSC-RK0272Human GPS1 Knockdown Cell Line-HeLaInquriy
CSC-RK0273Human GPX8 Knockdown Cell Line-HeLaInquriy
CSC-RK0274Human EPAS1 Knockdown Cell Line-HeLaInquriy
CSC-RK0275Human ELAVL1 Knockdown Cell Line-HeLaInquriy
CSC-RK0276Human NF1 Knockdown Cell Line-HeLaInquriy
CSC-RK0277Human NFE2L2 Knockdown Cell Line-HeLaInquriy
CSC-RK0278Human NUDCD1 Knockdown Cell Line-HeLaInquriy
CSC-RK0279Human OGG1 Knockdown Cell Line-HeLaInquriy
CSC-RK0280Human CDKN1A Knockdown Cell Line-HeLaInquriy
CSC-RK0281Human PIK3CD Knockdown Cell Line-HeLaInquriy
CSC-RK0282Human PRKCA Knockdown Cell Line-HeLaInquriy
CSC-RK0283Human PRDX1 Knockdown Cell Line-HeLaInquriy
CSC-RK0284Human PRDX2 Knockdown Cell Line-HeLaInquriy
CSC-RK0285Human RAD54B Knockdown Cell Line-HeLaInquriy
CSC-RK0286Human RHOQ Knockdown Cell Line-HeLaInquriy
CSC-RK0287Human SEPT9 Knockdown Cell Line-HeLaInquriy
CSC-RK0288Human SIRT1 Knockdown Cell Line-HeLaInquriy
CSC-RK0289Human SRI Knockdown Cell Line-HeLaInquriy
CSC-RK0290Human SSX2IP Knockdown Cell Line-HeLaInquriy
CSC-RK0291Human STAT1 Knockdown Cell Line-HeLaInquriy
CSC-RK0292Human THTPA Knockdown Cell Line-HeLaInquriy
CSC-RK0293Human TMSB4X Knockdown Cell Line-HeLaInquriy
CSC-RK0294Human TNFRSF10B Knockdown Cell Line-HeLaInquriy
CSC-RK0296Human TXNRD2 Knockdown Cell Line-HeLaInquriy
CSC-RK0297Human USP7 Knockdown Cell Line-HeLaInquriy
CSC-RK0298Human USP9X Knockdown Cell Line-HeLaInquriy
CSC-RK0041Human HDAC1 Knockdown Cell Line-HeLaInquriy
CSC-RK0042Human MAPK1 Knockdown Cell Line-HeLaInquriy
CSC-RK0043Human HMG1L1 Knockdown Cell Line-HeLaInquriy
CSC-RK0044Human PARG Knockdown Cell Line-HeLaInquriy
CSC-RT0120FHL1 Knockout Cell Line-HelaInquriy
CSC-RT0123PML Knockout Cell Line-HelaInquriy
CSC-RT0129LTN1 Knockout Cell Line-HelaInquriy
CSC-RT0132CCND3 Knockout Cell Line-HeLaInquriy
CSC-RT0133MPI Knockout Cell Line-HeLaInquriy
CSC-RT0134CD276 Knockout Cell Line-HeLaInquriy
CSC-RT0135TSC22D3 Knockout Cell Line-HeLaInquriy
CSC-RT0136HINT1 Knockout Cell Line-HeLaInquriy
CSC-RT0137OAZ1 Knockout Cell Line-HeLaInquriy
CSC-RT0138USP14 Knockout Cell Line-HeLaInquriy
CSC-RT0139FADD Knockout Cell Line-HeLaInquriy
CSC-RT0140SIR2L3 Knockout Cell Line-HeLaInquriy
CSC-RT0141ATG16L1 Knockout Cell Line-HeLaInquriy
CSC-RT0982Human SNX12 Knockout Cell Line-HeLaInquriy
CSC-RT0983Human NUPR1 Knockout Cell Line-HeLaInquriy
CSC-RT0984Human PAPSS2 Knockout Cell Line-HeLaInquriy
CSC-RT0985Human TFAP2A Knockout Cell Line-HeLaInquriy
CSC-RT0986Human CDC42EP4 Knockout Cell Line-HeLaInquriy
CSC-RT0987Human COL5A1 Knockout Cell Line-HeLaInquriy
CSC-RT0988Human LIMCH1 Knockout Cell Line-HeLaInquriy
CSC-RT0989Human TPM1 Knockout Cell Line-HeLaInquriy
CSC-RT0990Human STEAP4 Knockout Cell Line-HeLaInquriy
CSC-RT0991Human ANXA3 Knockout Cell Line-HeLaInquriy
CSC-RT0992Human ERGIC2 Knockout Cell Line-HeLaInquriy
CSC-RT0994Human PDK4 Knockout Cell Line-HeLaInquriy
CSC-RT0995Human HIST1H1C Knockout Cell Line-HeLaInquriy
CSC-RT0996Human GPSM2 Knockout Cell Line-HeLaInquriy
CSC-RT0997Human CLPX Knockout Cell Line-HeLaInquriy
CSC-RT0999Human FAM20B Knockout Cell Line-HeLaInquriy
CSC-RT1002Human SLCO4A1 Knockout Cell Line-HeLaInquriy
CSC-RK0299Human WRAP73 Knockdown Cell Line-HeLaInquriy
CSC-RK0300Human XIAP Knockdown Cell Line-HeLaInquriy
CSC-RT0495TP53 Knockout Cell Line-HeLaInquriy
CSC-RT0496Human VIM Knockout Cell Line-HeLaInquriy
CSC-RT0497Human KRT7 Knockout Cell Line-HeLaInquriy
CSC-RT0498Human CD68 Knockout Cell Line-HeLaInquriy
CSC-RT0499Human KRT8 Knockout Cell Line-HeLaInquriy
CSC-RT0500Human CTNND1 Knockout Cell Line-HeLaInquriy
CSC-RT0501Human MSH6 Knockout Cell Line-HeLaInquriy
CSC-RT0502Human CCND1 Knockout Cell Line-HeLaInquriy
CSC-RT0503Human LGALS3 Knockout Cell Line-HeLaInquriy
CSC-RT0504Human VEGFA Knockout Cell Line-HeLaInquriy
CSC-RT0505Human SDC1 Knockout Cell Line-HeLaInquriy
CSC-RT0506Human ERCC1 Knockout Cell Line-HeLaInquriy
CSC-RT0507Human MET Knockout Cell Line-HeLaInquriy
CSC-RT0508Human IDH1 Knockout Cell Line-HeLaInquriy
CSC-RT0509Human CALD1 Knockout Cell Line-HeLaInquriy
CSC-RT0510Human ALPP Knockout Cell Line-HeLaInquriy
CSC-RT0511Human MVP Knockout Cell Line-HeLaInquriy
CSC-RT0512Human CDKN1C Knockout Cell Line-HeLaInquriy
CSC-RT0513Human ABCC3 Knockout Cell Line-HeLaInquriy
CSC-RT0514Human TFE3 Knockout Cell Line-HeLaInquriy
CSC-RT0515Human CD44 Knockout Cell Line-HeLaInquriy
CSC-RT0516Human MDM2 Knockout Cell Line-HeLaInquriy
CSC-RT0517Human HIF1A Knockout Cell Line-HeLaInquriy
CSC-RT0518Human KRAS Knockout Cell Line-HeLaInquriy
CSC-RT0519Human COL4A1 Knockout Cell Line-HeLaInquriy
CSC-RT0520Human CDKN1B Knockout Cell Line-HeLaInquriy
CSC-RT0521Human TUBB3 Knockout Cell Line-HeLaInquriy
CSC-RT0522Human KRT10 Knockout Cell Line-HeLaInquriy
CSC-RT0523Human LAMA1 Knockout Cell Line-HeLaInquriy
CSC-RT0524Human CASP3 Knockout Cell Line-HeLaInquriy
CSC-RT0525Human FSCN1 Knockout Cell Line-HeLaInquriy
CSC-RT0526Human EZH2 Knockout Cell Line-HeLaInquriy
CSC-RT0527Human PSCA Knockout Cell Line-HeLaInquriy
CSC-RT0528Human BAX Knockout Cell Line-HeLaInquriy
CSC-RT0529Human CAV1 Knockout Cell Line-HeLaInquriy
CSC-RT0530Human HSPB1 Knockout Cell Line-HeLaInquriy
CSC-RT0531Human S100A4 Knockout Cell Line-HeLaInquriy
CSC-RT0532Human STMN1 Knockout Cell Line-HeLaInquriy
CSC-RT0533Human ICAM1 Knockout Cell Line-HeLaInquriy
CSC-RT0534Human FOLR1 Knockout Cell Line-HeLaInquriy
CSC-RT0535Human CD9 Knockout Cell Line-HeLaInquriy
CSC-RT0536Human CCNB1 Knockout Cell Line-HeLaInquriy
CSC-RT0537Human TIMP1 Knockout Cell Line-HeLaInquriy
CSC-RT0538Human TLE1 Knockout Cell Line-HeLaInquriy
CSC-RT0539Human ERBB2 Knockout Cell Line-HeLaInquriy
CSC-RT0540Human AMACR Knockout Cell Line-HeLaInquriy
CSC-RT0541Human RB1 Knockout Cell Line-HeLaInquriy
CSC-RT0542Human ARF1 Knockout Cell Line-HeLaInquriy
CSC-RT0543Human NME2 Knockout Cell Line-HeLaInquriy
CSC-RT0544Human TNFRSF10A Knockout Cell Line-HeLaInquriy
CSC-RT0545Human BRE Knockout Cell Line-HeLaInquriy
CSC-RT0546Human EZR Knockout Cell Line-HeLaInquriy
CSC-RT0547Human PIK3C2A Knockout Cell Line-HeLaInquriy
CSC-RT0548Human CDKN2C Knockout Cell Line-HeLaInquriy
CSC-RT0549Human EPHA2 Knockout Cell Line-HeLaInquriy
CSC-RT0550Human ASS1 Knockout Cell Line-HeLaInquriy
CSC-RT0551Human ASL Knockout Cell Line-HeLaInquriy
CSC-RT0552Human BMP2 Knockout Cell Line-HeLaInquriy
CSC-RT0553Human YBX1 Knockout Cell Line-HeLaInquriy
CSC-RT0554Human DKK1 Knockout Cell Line-HeLaInquriy
CSC-RT0555Human TACC2 Knockout Cell Line-HeLaInquriy
CSC-RT0556Human LAMB3 Knockout Cell Line-HeLaInquriy
CSC-RT0557Human PHGDH Knockout Cell Line-HeLaInquriy

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