HeLa Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	A derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in a number of subsequently derived lines, due to poor culture techniques. Susceptible to Poliovirus type I and adenovirus type 3.
Tissue of Origin	Human cervix carcinoma
Age	31 years adult
Gender	Female
Ethnicity	Black
Cell Type	Epithelial
Product Format	Frozen
Growth Mode	Adherent
Applications	These cells are a suitable transfection host. And the cell line can be used to screen for Escherichia coli strains with invasive potential.
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Volumes are given for a 75 cm² flask. Proportionally increase or reduce the amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	The ratio of 1:2 to 1:6 is recommended.
Fluid Renewal	2 to 3 times per week
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Culture Temperature	37°C
Atmosphere	Air, 95%; carbon dioxide (CO₂), 5%

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Cat.No.	Product Name	Price
CLKO-0001	VIM KO Cell Lysate-HeLa	Inquiry
CLKO-0002	KRT7 KO Cell Lysate-HeLa	Inquiry
CLKO-0003	CD68 KO Cell Lysate-HeLa	Inquiry
CLKO-0004	KRT8 KO Cell Lysate-HeLa	Inquiry
CLKO-0005	CTNND1 KO Cell Lysate-HeLa	Inquiry
CLKO-0006	MSH6 KO Cell Lysate-HeLa	Inquiry
CLKO-0007	CCND1 KO Cell Lysate-HeLa	Inquiry
CLKO-0008	LGALS3 KO Cell Lysate-HeLa	Inquiry
CLKO-0009	VEGFA KO Cell Lysate-HeLa	Inquiry
CLKO-0010	SDC1 KO Cell Lysate-HeLa	Inquiry
CLKO-0011	ERCC1 KO Cell Lysate-HeLa	Inquiry
CLKO-0012	MET KO Cell Lysate-HeLa	Inquiry
CLKO-0013	IDH1 KO Cell Lysate-HeLa	Inquiry
CLKO-0014	CALD1 KO Cell Lysate-HeLa	Inquiry
CLKO-0015	ALPP KO Cell Lysate-HeLa	Inquiry

* For research use only. Not intended for any clinical use.

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