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RNA sequencing (RNA-seq) is a high-throughput sequencing technology used to analyze the transcriptome, which is a set of RNA molecules expressed in cells, tissues or organisms. RNA-seq provides a comprehensive and accurate method for studying gene expression, alternative splicing, non-coding RNA and RNA editing events. RNA-seq sample preparation is the process of isolating, purifying RNA molecules, and converting them into a library suitable for sequencing using next-generation sequencing (NGS) technology. Separate RNA from the sample and fragment it into smaller fragments. Then convert the fragment into cDNA and add an adapter to the end of the fragment. Then the library was amplified by polymerase chain reaction (PCR) and sequenced by NGS technology.
Importance RNA-seq is a powerful transcriptome analysis technology, and at the same time, it carries out fine sample processing before library sequencing. Obtaining pure and high-quality RNA samples is crucial for the successful preparation of RNA-seq samples. Careful consideration should be given to the storage of tissues that extract RNA, as RNA is more unstable than DNA. For long-term storage of RNA, a temperature of -80°C is usually recommended to better prevent degradation and ensure molecular integrity.
Creative Biogene provides a complete set of streamlined RNA-seq sample preparation kits, providing reagents and protocols for separating, purifying RNA molecules, and converting RNA molecules into libraries suitable for sequencing using next-generation sequencing (NGS) technology. These kits provide a standardized and optimized method for RNA-seq sample preparation, reducing variability between experiments and ensuring high-quality results. Please browse our RNA-seq Sample Preparation Kits collection to find your desired one.
| Cat.No. | Product Name | Price |
|---|---|---|
| DSPK-09 | Universal mRNA-seq Sample Prep Kit | Inquiry |
| DSPK-10 | Stranded mRNA-seq Sample Prep Kit | Inquiry |
| DSPK-11 | Whole RNA-seq Sample Prep Kit | Inquiry |
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