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Exosomes are 30 to 150 nm membrane-wrapped extracellular vesicles found in biological fluids and cell cultures. Generated from late endosomes, they carry DNA, RNA, lipids, and proteins, including CD9, CD63, and CD81. Exosomes facilitate cell-to-cell communication, influencing immune response and antigen presentation. Creative Biogene offers lentivirus particles with exosome biomarkers for studying release, uptake, and biodistribution mechanisms efficiently. Explore exosome applications as biomarkers and therapeutics with our recombinant lentiviruses.
Key Features of Our Exosome Marker Lentiviral Particles
Exosome Marker Lentiviral Particles Product List
The exosome marker lentiviruses allow real-time monitoring of exosome biogenesis, uptake, and biodistribution through the integration of traceable reporters. Quantifying reporter expression provides sensitive measurements of exosome release from transduced cells. Fluorescent labeling enables exosome visualization. The principle involves lentiviral integration of CD63/CD81-reporter fusions that incorporate into secreted exosomes. The customizable vectors provide innovative tools to elucidate exosome functions and explore diagnostic/therapeutic potential. Perhaps your research work requires tools in the following directions:
Case Study 1
HIV-1 establishes a persistent latent infection. Managing HIV-1 with combination antiretroviral therapy (cART) entails life-shortening side effects and the emergence of drug-resistant strains. Researchers developed an HIV-1 promoter-targeting Zinc Finger Protein (ZFP-362) fused to DNA methyltransferase 3 A for stable epigenetic repression. Exosomes loaded with this HIV-1 repressor suppress virus expression via DNA methylation in humanized NSG mouse models, offering a potential systemic delivery platform for HIV-1 therapy.
Figure 1. Researchers employed lentiviruses to transduce hTERT-immortalized mesenchymal stem cells with nLuc (Control), ZFP, or ZPAMt payloads. Co-culturing ZPAMt exosome-producing MSCs with CHI-Ju cells resulted in the repression of HIV-1 Gag mRNA. (Shrivastava S, et al., 2021)
Case Study 2
Exosomes transport factors promoting tumor growth in glioblastoma (GBM), where fusion genes, potent driver mutations in neoplasia, play a crucial role in tumorigenesis. Researchers utilized lentiviral vectors to investigate the role of exosomes in glioblastoma (GBM) progression, focusing on cells with the PTPRZ1–MET fusion (ZM fusion). Exosomes from ZM fusion cells (ZM exosomes) displayed enhanced pro-oncogenic characteristics and were internalized by recipient cells. ZM exosomes induced MET proto-oncogene expression, phosphorylation of MET, altered gene expression, and promoted aggressive GBM phenotypes, including migration, invasion, neurosphere growth, angiogenesis, and temozolomide resistance. These findings underscore the pivotal role of ZM fusion in mediating the aggressive nature of GBM via exosome-mediated intercellular communication, suggesting potential implications for gene fusion-based therapies in GBM management.
Figure 2. Researchers established U87/ZM cells expressing red fluorescent protein (RFP)-tagged exosomal marker CD63 (U87/ZM/RFP-CD63) to visualize exosome transfer. (Zeng A L, et al., 2017)
Q: Which common exosome markers are incorporated into these lentiviral particles for visualization and identification?
A: Common exosome markers, such as CD9, CD63, and CD81, are often incorporated into these particles to label and identify exosomes in biological samples.
Q: How do Exosome Marker Lentiviral Particles contribute to the isolation and purification of exosomes?
A: These particles enable the visualization of exosomes, facilitating their isolation and purification from complex biological samples, leading to more accurate and efficient exosome research.
Q: How can researchers use the labeled exosomes in downstream applications, such as functional studies or biomarker discovery?
A: Labeled exosomes can be used in functional studies to explore their roles in recipient cells and contribute to biomarker discovery by identifying specific exosomal markers associated with disease states.
| Cat.No. | Product Name | Price |
|---|---|---|
| LVE00995Z | EF1a-CD9-tdTomato Lentivirus | Inquiry |
| LVE00996Z | EF1a-CD63-tdTomato Lentivirus | Inquiry |
| LVE00997Z | EF1a-CD81-tdTomato Lentivirus | Inquiry |
| LVE00998Z | EF1a-FLAG-mCherry-CD63 Lentivirus | Inquiry |
| LVE00999Z | EF1a-FLAG-Luc-CD63 Lentivirus | Inquiry |
| LVE01000Z | EF1a-Halo-CD63 Lentivirus | Inquiry |
| LVE01001Z | EF1a-Avi-CD63 Lentivirus | Inquiry |
| LVE01002Z | CMV-CD63-GFP-FLAG Lentivirus | Inquiry |
| LVE01003Z | CMV-CD81-GFP-FLAG Lentivirus | Inquiry |
| LVE01004Z | CMV-CD63-mCherry-FLAG Lentivirus | Inquiry |
| LVE01005Z | CMV-CD81-mCherry-FLAG Lentivirus | Inquiry |
| LVE01006Z | CMV-CD9-mCherry-FLAG Lentivirus | Inquiry |
| LVE01007Z | CMV-FLAG-GFP-CD63 Lentivirus | Inquiry |
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