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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H358, also known as H-358 or NCIH358, was isolated from primary bronchoalveolar carcinoma collected before treatment from a white male. Ultrastructural studies of this non-small cell lung cancer (NSCLC) revealed the presence of granules characteristic of Clara cells. NCI-H358 cells are particularly relevant to cancer research in NSCLC, particularly exploring the biology and treatment of lung adenocarcinoma. This cell line is critical for studying the effectiveness of therapies targeting the epidermal growth factor receptor (EGFR), as EGFR mutations are an important focus of NSCLC treatment. Additionally, NCI-H358 cells are valuable for studying the role of KRAS mutations, which are prevalent in lung cancer and known to drive oncogenic activity. Study of these mutations in NCI-H358 cells helps elucidate molecular pathways associated with lung cancer progression and treatment resistance. |
| Tissue | Lung |
| Disease | Minimally invasive lung adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | Age unspecified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Pharmacological studies 3. Gene function studies 4. Toxicology test 5. In vitro modelling 6. Molecular biology studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | NCI-H358 does not express UDP-glucuronosyltransferase, but expresses glutathione-S-transferase and phenol sulfotransferase. Expression of the major surfactant-associated protein SP-A protein and RNA was detected. SP-B and SP-C RNA are not expressed. The NCI-H358 cell line contains homozygous deletion of the major tumor suppressor p53. The H358 lung cancer cell line is also used to evaluate the potential of novel therapeutic approaches. |
| Characteristics | |
| Protein Expression | UGT -, GST +, PST +, p53 - |
| Mutational Profile | p53 homozygously deleted |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. 2. Then, completely cover the cells with Accutase. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI 1640 + 2 mM Glutamine + 5-10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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