At Creative Biogene, we leverage over a decade of expertise in molecular biology to provide a comprehensive and reliable plasmid DNA preparation platform. Our services support global research institutions, biotechnology companies, and pharmaceutical enterprises, offering end-to-end solutions ranging from basic research to preclinical and GMP-grade applications.
Our team has completed over 5,000 plasmid preparation projects, handling conventional plasmids as well as those with complex structures, high GC content, repetitive sequences, and unstable elements. Dedicated technical support ensures expert guidance throughout project design, execution, and troubleshooting.
Specialized Platform for Complex Plasmids
Creative Biogene has optimized processes for challenging plasmid constructs:
01 AAV ITR Stability
Specialized host strains, optimized culture conditions, and multi-level verification (restriction analysis, sequencing, functional assays) ensure >95% ITR integrity.
02 Long poly(A) Sequences
Directed cloning strategies and verification steps maintain >98% sequence fidelity.
03 High GC Content Regions
Optimized amplification and sequencing strategies prevent secondary structure formation and ensure sequence accuracy.
04 Large Plasmids (>20 kb)
Low-copy plasmids are extracted using gentle lysis methods and specialized purification for high recovery.
Research-Grade Plasmid DNA
Designed for general molecular biology applications, providing a cost-effective solution with reliable quality. Suitable for cloning, PCR, sequencing, restriction analysis, and educational experiments.
Transfection-Grade Plasmid DNA
Optimized for mammalian cell transfection, viral vector packaging, and recombinant protein expression. Ideal for transient and stable transfection, reporter assays, and gene function studies.
Produced under full regulatory compliance for preclinical and clinical applications. Suitable for gene therapy vectors, DNA vaccines, CAR-T cell therapy, virus production, and early-phase clinical studies.
Our plasmid DNA services are supported by a multi-tiered quality control system that ensures consistency and reliability at every stage. From strain authentication to final product release, we implement stringent quality measures using advanced techniques such as agarose gel electrophoresis, HPLC, qPCR, and NGS. All quality control processes adhere to clearly defined acceptance criteria, with no compromise on standards.
Research-Grade
Transfection-Grade
GMP-Grade
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Concentration & Purity | UV Spectrophotometry | A260/A280: 1.8–2.0; A260/A230 ≥ 2.0 |
| Integrity | Agarose Gel Electrophoresis | Clear main band, no obvious degradation |
| Restriction Digest | Restriction Enzyme Digestion | Fragment sizes match expectation |
| Sequence Verification | Sanger Sequencing (Optional) | Insert region 100% correct |
| Residual Host DNA | Agarose Gel | Not detectable |
| Residual RNA | Agarose Gel | Not detectable |
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Concentration & Purity | UV Spectrophotometry | A260/A280: 1.8–2.0; A260/A230 ≥ 2.2 |
| Supercoiled Ratio | HPLC / Capillary Electrophoresis | >80% |
| Endotoxin | Chromogenic LAL Assay | <0.1 EU/μg |
| Integrity | Agarose Gel Electrophoresis | Clear main band, supercoiled dominant |
| Restriction Digest | Restriction Enzyme Digestion | Fragment sizes match expectation |
| Sequence Verification | Sanger Sequencing (Optional) | Insert region 100% correct |
| Residual Host DNA | qPCR | <1% |
| Residual RNA | Fluorescence Assay | <1% |
| Sterility | Direct Inoculation | No microbial growth |
| Mycoplasma Detection | PCR (Optional) | Negative |
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Identity | Restriction Enzyme Analysis | Matches expected pattern |
| Identity | Full Sequence Verification (NGS) | 100% sequence match |
| Concentration | UV A260 | Within specification |
| Purity | UV A260/A280 | 1.8–2.0 |
| Purity | HPLC | >95% |
| Supercoiled Ratio | HPLC / CE | >85% |
| Endotoxin | Chromogenic LAL Assay | <0.01 EU/μg |
| Residual Host DNA | qPCR | <0.1% |
| Residual Host Protein | BCA / ELISA | <0.1% |
| Residual RNA | Fluorescence Assay | <0.5% |
| Bioburden | Microbial Limit Test | <1 CFU/mg |
| Sterility | Membrane Filtration | Sterile |
| Mycoplasma Test | Culture & PCR | Negative |
| Recoverable Yield | Gravimetric | >90% of labeled amount |
| Solubility | Redissolution Test | Fully soluble |
Case Study 1
Researchers investigated cold-stress responses in rice by creating transgenic japonica lines to overexpress or knock down pri-miR1850, miR1850.1, and miR1850.2, using pCAMBIA3301 vectors supplied by Creative Biogene, CRISPR-Cas9 cassettes, and amiRNA/STTM constructs to study the miR1850–NPR3 module's effects on cold tolerance, disease resistance, and grain yield.
Figure 1. Rice pri-miR1850 and its mature miRNAs regulate cold stress responses, affecting gene expression, seedling survival, and reproductive traits. (Shen Y, et al., 2025)
Case Study 2
To investigate the ABI1 protein interactome, researchers constructed the MSCV‐TurboID‐Linker‐ABI1‐IRES‐GFP plasmid. This construct, synthesized in collaboration with Creative Biogene, enabled proximity-dependent biotin labeling of ABI1 in mammalian cells. Following transfection, biotinylated proteins were identified via mass spectrometry. The study successfully detected 212 ABI1 proximal interactors, including WAVE2 complex components and proteins associated with the TAK1‐IKK pathway.
Figure 2. Development of TurboID-based ABI1 proximity labeling tools and corresponding NIH/3T3 cell lines. (Petersen M, et al., 2023)
Our streamlined workflow ensures clarity and efficiency:
1Discuss requirements and receive proposal within 48 hours.
2Confirm agreement, provide materials, and plan the project.
3Conduct strain validation, fermentation, plasmid extraction, and intermediate QC.
4Perform final testing, analyze data, compile reports, and release product.
5Ship product with documentation and provide ongoing technical support.
Whether for microgram-scale exploratory research or gram-scale clinical-grade plasmids, we provide consistent quality, professional service, and a reliable supply chain. Choosing Creative Biogene ensures a trusted scientific partner dedicated to advancing research outcomes.
Q: How do I select the appropriate plasmid preparation grade?
A: Research-grade for basic experiments, transfection-grade for cell transfection and viral packaging, and GMP-grade for preclinical and clinical applications. Our technical team can recommend the optimal grade based on your needs.
Q: What types of plasmids can you process?
A: We handle high- and low-copy plasmids, sizes 2 kb–50 kb+, and plasmids with unstable sequences (e.g., ITRs, poly(A) regions). Please consult for special constructs.
Q: What documentation is provided for GMP-grade plasmids?
A: Complete GMP documentation, including batch production and QC records, release certificates, quality standards, analytical validation reports, and stability study data to support regulatory submissions.
Q: Why is endotoxin important, and how should I choose?
A: Endotoxin affects transfection efficiency, cell viability, and in vivo immune responses. Recommended levels: <10 EU/μg for bacterial transformation, <1 EU/μg for cell transfection, <0.1 EU/μg for in vivo experiments, and <0.01 EU/μg for clinical applications.
Q: Why is the supercoiled form important?
A: Supercoiled plasmids have higher transfection efficiency and stability. Research-grade >70%, transfection-grade >80%, GMP-grade >85%.
Contact our expert team to discuss your project needs and ensure the highest quality plasmid DNA for your research or therapeutic development.
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