Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
Ready-to-Use | High Titer | Versatile Applications
Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
Stable | Scalable | Customizable
Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
Precise | High Yield | Tailored Solutions
Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
Authentic | Versatile | Accelerated
Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
Validated | Reliable | Comprehensive Collection
Ready-to-use clones for streamlined research and development.
Kits
Complete | Convenient | High Sensitivity
Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
Purified | Stable | Efficient
Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
Highly Specific | Robust | Versatile
Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
Enhancing | Synergistic | Effective
Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
Innovative | Reliable | High-Precision
Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
Stable Cell Line Generation
Reliable | Scalable | Customizable
Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
Innovative | Comprehensive | Efficient
Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
Versatile | High-Yield | Safe
Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
Custom Antibody Service
Precise | Flexible | Efficient
End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
Antibody-Drug Conjugation Service
Integrated | Controlled | Translational
Comprehensive solutions covering design, development, and validation to ensure conjugated drugs with consistent quality and clinical potential.
Protein Degrader Service
Efficient | High-Precision | Advanced Therapeutics
Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
Accurate | Flexible | High-Quality
Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
Custom RNA Service
Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
Comprehensive | High-throughput | Accurate
Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
Precise | Efficient | Targeted
Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
Precise | Scalable | Customizable
Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
Biosafety Testing Service
Reliable | Comprehensive | Regulated
Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
Plant Genetic Modification Service
Advanced | Sustainable | Tailored
Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
Plant-based Protein Production Service
Efficient | Scalable | Customizable
Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
Innovative | Fast | Cost-Effective
Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
Comprehensive | Accurate | Regulatory-compliant
Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
Rapid | Precise | Scalable
Balancing accuracy, accessibility, affordability, and rapid detection to safeguard public health and strengthen global response to infectious diseases.
cGMP Cell Line Development
Reliable | Scalable | Industry-leading
Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
Efficient | Scalable | Precise
Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
GMP Plasmid Production
High Quality | Scalable | Regulatory-compliant
Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
Scalable | High Yield | Quality-driven
Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
Innovative | Precision | Transformative
AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
Next-Generation | Targeted | Efficient
AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
Smart | Efficient | Tailored
High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
Predictive | Efficient | Insightful
Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
Innovative | Targeted | Accelerated
Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
At Creative Biogene, we leverage over a decade of expertise in molecular biology to provide a comprehensive and reliable plasmid DNA preparation platform. Our services support global research institutions, biotechnology companies, and pharmaceutical enterprises, offering end-to-end solutions ranging from basic research to preclinical and GMP-grade applications.
Our team has completed over 5,000 plasmid preparation projects, handling conventional plasmids as well as those with complex structures, high GC content, repetitive sequences, and unstable elements. Dedicated technical support ensures expert guidance throughout project design, execution, and troubleshooting.
Specialized Platform for Complex Plasmids
Creative Biogene has optimized processes for challenging plasmid constructs:
01 AAV ITR Stability
Specialized host strains, optimized culture conditions, and multi-level verification (restriction analysis, sequencing, functional assays) ensure >95% ITR integrity.
02 Long poly(A) Sequences
Directed cloning strategies and verification steps maintain >98% sequence fidelity.
03 High GC Content Regions
Optimized amplification and sequencing strategies prevent secondary structure formation and ensure sequence accuracy.
04 Large Plasmids (>20 kb)
Low-copy plasmids are extracted using gentle lysis methods and specialized purification for high recovery.
Research-Grade Plasmid DNA
Designed for general molecular biology applications, providing a cost-effective solution with reliable quality. Suitable for cloning, PCR, sequencing, restriction analysis, and educational experiments.
Transfection-Grade Plasmid DNA
Optimized for mammalian cell transfection, viral vector packaging, and recombinant protein expression. Ideal for transient and stable transfection, reporter assays, and gene function studies.
Produced under full regulatory compliance for preclinical and clinical applications. Suitable for gene therapy vectors, DNA vaccines, CAR-T cell therapy, virus production, and early-phase clinical studies.
Our plasmid DNA services are supported by a multi-tiered quality control system that ensures consistency and reliability at every stage. From strain authentication to final product release, we implement stringent quality measures using advanced techniques such as agarose gel electrophoresis, HPLC, qPCR, and NGS. All quality control processes adhere to clearly defined acceptance criteria, with no compromise on standards.
Research-Grade
Transfection-Grade
GMP-Grade
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Concentration & Purity | UV Spectrophotometry | A260/A280: 1.8–2.0; A260/A230 ≥ 2.0 |
| Integrity | Agarose Gel Electrophoresis | Clear main band, no obvious degradation |
| Restriction Digest | Restriction Enzyme Digestion | Fragment sizes match expectation |
| Sequence Verification | Sanger Sequencing (Optional) | Insert region 100% correct |
| Residual Host DNA | Agarose Gel | Not detectable |
| Residual RNA | Agarose Gel | Not detectable |
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Concentration & Purity | UV Spectrophotometry | A260/A280: 1.8–2.0; A260/A230 ≥ 2.2 |
| Supercoiled Ratio | HPLC / Capillary Electrophoresis | >80% |
| Endotoxin | Chromogenic LAL Assay | <0.1 EU/μg |
| Integrity | Agarose Gel Electrophoresis | Clear main band, supercoiled dominant |
| Restriction Digest | Restriction Enzyme Digestion | Fragment sizes match expectation |
| Sequence Verification | Sanger Sequencing (Optional) | Insert region 100% correct |
| Residual Host DNA | qPCR | <1% |
| Residual RNA | Fluorescence Assay | <1% |
| Sterility | Direct Inoculation | No microbial growth |
| Mycoplasma Detection | PCR (Optional) | Negative |
| QC Item | Method | Acceptance Criteria |
| Appearance | Visual Inspection | Colorless and transparent, no precipitation |
| Identity | Restriction Enzyme Analysis | Matches expected pattern |
| Identity | Full Sequence Verification (NGS) | 100% sequence match |
| Concentration | UV A260 | Within specification |
| Purity | UV A260/A280 | 1.8–2.0 |
| Purity | HPLC | >95% |
| Supercoiled Ratio | HPLC / CE | >85% |
| Endotoxin | Chromogenic LAL Assay | <0.01 EU/μg |
| Residual Host DNA | qPCR | <0.1% |
| Residual Host Protein | BCA / ELISA | <0.1% |
| Residual RNA | Fluorescence Assay | <0.5% |
| Bioburden | Microbial Limit Test | <1 CFU/mg |
| Sterility | Membrane Filtration | Sterile |
| Mycoplasma Test | Culture & PCR | Negative |
| Recoverable Yield | Gravimetric | >90% of labeled amount |
| Solubility | Redissolution Test | Fully soluble |
Case Study 1
Researchers investigated cold-stress responses in rice by creating transgenic japonica lines to overexpress or knock down pri-miR1850, miR1850.1, and miR1850.2, using pCAMBIA3301 vectors supplied by Creative Biogene, CRISPR-Cas9 cassettes, and amiRNA/STTM constructs to study the miR1850–NPR3 module's effects on cold tolerance, disease resistance, and grain yield.
Figure 1. Rice pri-miR1850 and its mature miRNAs regulate cold stress responses, affecting gene expression, seedling survival, and reproductive traits. (Shen Y, et al., 2025)
Case Study 2
To investigate the ABI1 protein interactome, researchers constructed the MSCV‐TurboID‐Linker‐ABI1‐IRES‐GFP plasmid. This construct, synthesized in collaboration with Creative Biogene, enabled proximity-dependent biotin labeling of ABI1 in mammalian cells. Following transfection, biotinylated proteins were identified via mass spectrometry. The study successfully detected 212 ABI1 proximal interactors, including WAVE2 complex components and proteins associated with the TAK1‐IKK pathway.
Figure 2. Development of TurboID-based ABI1 proximity labeling tools and corresponding NIH/3T3 cell lines. (Petersen M, et al., 2023)
Our streamlined workflow ensures clarity and efficiency:
1Discuss requirements and receive proposal within 48 hours.
2Confirm agreement, provide materials, and plan the project.
3Conduct strain validation, fermentation, plasmid extraction, and intermediate QC.
4Perform final testing, analyze data, compile reports, and release product.
5Ship product with documentation and provide ongoing technical support.
Whether for microgram-scale exploratory research or gram-scale clinical-grade plasmids, we provide consistent quality, professional service, and a reliable supply chain. Choosing Creative Biogene ensures a trusted scientific partner dedicated to advancing research outcomes.
Q: How do I select the appropriate plasmid preparation grade?
A: Research-grade for basic experiments, transfection-grade for cell transfection and viral packaging, and GMP-grade for preclinical and clinical applications. Our technical team can recommend the optimal grade based on your needs.
Q: What types of plasmids can you process?
A: We handle high- and low-copy plasmids, sizes 2 kb–50 kb+, and plasmids with unstable sequences (e.g., ITRs, poly(A) regions). Please consult for special constructs.
Q: What documentation is provided for GMP-grade plasmids?
A: Complete GMP documentation, including batch production and QC records, release certificates, quality standards, analytical validation reports, and stability study data to support regulatory submissions.
Q: Why is endotoxin important, and how should I choose?
A: Endotoxin affects transfection efficiency, cell viability, and in vivo immune responses. Recommended levels: <10 EU/μg for bacterial transformation, <1 EU/μg for cell transfection, <0.1 EU/μg for in vivo experiments, and <0.01 EU/μg for clinical applications.
Q: Why is the supercoiled form important?
A: Supercoiled plasmids have higher transfection efficiency and stability. Research-grade >70%, transfection-grade >80%, GMP-grade >85%.
Contact our expert team to discuss your project needs and ensure the highest quality plasmid DNA for your research or therapeutic development.
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