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Transfection alters the genetic makeup of eukaryotic cells by introducing foreign nucleic acids, including DNA, RNA, and small noncoding RNAs such as siRNA, shRNA, and miRNA. Stable transfection results in sustained, long-term expression, whereas expression is eventually lost following transient transfection as the host cell replicates. Scientists apply stable transfection to long-term and large-scale genetic and pharmacological studies. Transient transfection can be used for short-term studies, such as studying the effects of gene knock-in or knock-down.
Transfection reagents are substances that facilitate the entry of nucleic acids into cells. They consist of a combination of various compounds that form complexes with nucleic acids and protect them from degradation in the extracellular environment. Different transfection reagents have unique chemical properties that interact with cellular components, allowing nucleic acid cargo to be delivered into the cell. Once inside the cell, the transfection complex must escape from the endosome and enter the cytoplasm, where genetic material can be transcribed into RNA and then translated into protein.
Researchers need to study gene function through gene overexpression (cDNA cloning), gene knockdown (siRNA or shRNA), and gene knockdown (CRISPR). Efficient delivery of these gene-associated products into cells is critical for their research. Transfection reagents from Creative Biogene are ideal for delivering all types of nucleic acids, including DNA, siRNA, miRNA, mRNA, viral RNA, and oligonucleotides, into eukaryotic cells. Our transfection reagents deliver nucleic acids into difficult-to-transfect cells, including cancer model cell lines, insect cells, and stem cells. Specific reagent protocols are optimized for efficiency, viability, and reproducibility in various cell types. Therefore, these transfection reagents have high efficiency and low toxicity in transfecting a variety of cell types.
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