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MicroRNAs (miRNAs) are short non-coding RNAs that function by destabilizing and/or inhibiting translation of target RNAs. miRNA-encoding genes are often transcribed as polycistronic pri-miRNAs, which are then cleaved by Drosha/Dgcr8 and Dicer nucleases. Mature miRNAs are molecules approximately 22 nucleotides long that typically regulate post-transcriptional gene expression by inhibiting translation or mRNA degradation. miRNAs are involved in nearly all cellular functions and regulate gene expression by binding to the 3' untranslated regions (3' UTRs) of target mRNAs. Abnormal expression of genes regulated by miRNAs has been found to be associated with cancer, cardiovascular disease, and a variety of other diseases.
Among various model organisms, mice have been widely used to study the role of miRNAs in gene regulation and their association with various diseases. The mouse genome is highly conserved with the human genome, making it an ideal model organism for studying a variety of biological processes.
miRNA-overexpressing cell lines are generated by transfecting cells with a plasmid or lentiviral vector encoding the miRNA sequence under the control of a promoter. miRNA-overexpressing cell lines offer several advantages for biomedical research compared to traditional methods of studying miRNAs through endogenous expression. First, miRNA-overexpressing cell lines provide a means to study the effects of miRNAs in a controlled environment. By manipulating the levels of miRNAs, researchers can study their downstream targets and pathways, revealing their biological functions. Second, miRNA-overexpressing cell lines can be used to validate miRNA targets and identify potential therapeutic targets. By introducing miRNAs into cells and examining the resulting changes in gene expression, researchers can identify new targets regulated by miRNAs of interest. Third, miRNA-overexpressing cell lines could facilitate the development of miRNA-based therapeutics. By testing the effects of miRNA overexpression or inhibition on cell viability, proliferation, and other biological processes, researchers can identify miRNAs with potential therapeutic applications.
Mouse miRNA-overexpressing cell lines have become valuable tools in biomedical research, providing a means to study miRNA function, validate miRNA targets, and develop miRNA-based therapeutics. By manipulating the levels of miRNAs, researchers can study their downstream targets and pathways, revealing their biological functions. Creative Biogene is developing stable cell lines overexpressing mouse miRNA precursors covered in the current miRbase. Our mouse miRNA overexpression stable cell lines are constructed by lentivirus transduction or non-viral plasmid transfection of vectors optimized by our scientists to enable the expression and maturation of miRNAs inside cells.
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